Amplification of viral RNA for the detection of dengue types 1 and 2 virus

Chia C. Pao; Ding Shyan Yao; Chieh Yu Lin; Chwan Chuen King

doi:10.1016/0163-4453(92)90842-T

Amplification of viral RNA for the detection of dengue types 1 and 2 virus

Chia C. Pao^*, Ding Shyan Yao, Chieh Yu Lin, Chwan Chuen King

^*Corresponding author for this work

Department of Biochemistry

Research output: Contribution to journal › Journal Article › peer-review

9 Scopus citations

Abstract

In vitro DNA amplification by means of the polymerase chain reaction (PCR) was used to amplify dengue types i and 2 viral genomes in cultured cells and in the serum of persons infected with dengue virus. Results of the present investigation suggest that the PCR method is type-specific in detecting dengue virus and has a detection sensitivity of less than 100 plaque-forming units (pfu) for both serotypes of the virus. The PCR method may be useful for detecting and typing dengue virus in clinical and epidemiological specimens.

Original language	English
Pages (from-to)	23-26,IN1-IN3,27-29
Journal	Journal of Infection
Volume	24
Issue number	1
DOIs	https://doi.org/10.1016/0163-4453(92)90842-T
State	Published - 01 1992

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10.1016/0163-4453(92)90842-T

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title = "Amplification of viral RNA for the detection of dengue types 1 and 2 virus",

abstract = "In vitro DNA amplification by means of the polymerase chain reaction (PCR) was used to amplify dengue types i and 2 viral genomes in cultured cells and in the serum of persons infected with dengue virus. Results of the present investigation suggest that the PCR method is type-specific in detecting dengue virus and has a detection sensitivity of less than 100 plaque-forming units (pfu) for both serotypes of the virus. The PCR method may be useful for detecting and typing dengue virus in clinical and epidemiological specimens.",

author = "Pao, {Chia C.} and Yao, {Ding Shyan} and Lin, {Chieh Yu} and King, {Chwan Chuen}",

year = "1992",

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AU - Pao, Chia C.

AU - Yao, Ding Shyan

AU - Lin, Chieh Yu

AU - King, Chwan Chuen

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Y1 - 1992/1

N2 - In vitro DNA amplification by means of the polymerase chain reaction (PCR) was used to amplify dengue types i and 2 viral genomes in cultured cells and in the serum of persons infected with dengue virus. Results of the present investigation suggest that the PCR method is type-specific in detecting dengue virus and has a detection sensitivity of less than 100 plaque-forming units (pfu) for both serotypes of the virus. The PCR method may be useful for detecting and typing dengue virus in clinical and epidemiological specimens.

AB - In vitro DNA amplification by means of the polymerase chain reaction (PCR) was used to amplify dengue types i and 2 viral genomes in cultured cells and in the serum of persons infected with dengue virus. Results of the present investigation suggest that the PCR method is type-specific in detecting dengue virus and has a detection sensitivity of less than 100 plaque-forming units (pfu) for both serotypes of the virus. The PCR method may be useful for detecting and typing dengue virus in clinical and epidemiological specimens.

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M3 - 文章

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JO - Journal of Infection

JF - Journal of Infection

IS - 1

ER -

Amplification of viral RNA for the detection of dengue types 1 and 2 virus

Abstract

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