An alkaloid-rich phytopharmaceutical prepared from Qing Dai against IL-17A-induced psoriasis

Chia Lin Lee; Chien Ming Wang; Ying Chyi Song; Chuan Teng Liu; Mei Yun Chu; Hung Rong Yen

doi:10.1016/j.jep.2023.116924

An alkaloid-rich phytopharmaceutical prepared from Qing Dai against IL-17A-induced psoriasis

Chia Lin Lee^*, Chien Ming Wang, Ying Chyi Song, Chuan Teng Liu, Mei Yun Chu, Hung Rong Yen^*

^*Corresponding author for this work

China Medical University Taichung

Research output: Contribution to journal › Journal Article › peer-review

4 Scopus citations

Abstract

Ethnopharmacological relevance: Psoriasis is a chronic inflammatory disease due to immune dysregulation that cannot be cured. The skin conditions of psoriasis negatively impact patients’ quality of life worldwide. Qing Dai (Indigo Naturalis), a traditional Chinese medicine (TCM) processed from Strobilanthes cusia is a clinical medicine used for psoriasis patient in Taiwan and the gene overexpression of interleukin (IL)-17A could be notably reduced in skin lesions after using Qing Dai ointment and its alkaloid ingredients. Aim of the study: To develop a potential anti-psoriatic phytopharmaceutical, an alkaloid-rich fraction named INM-A was prepared from Qing Dai. The chemical profile and anti-psoriatic activity of INM-A were analyzed and evaluated to define its in vitro mechanism and in vivo efficacy for psoriasis therapy. Materials and methods: Dowex® 50WX4 hydrogen form resin was used for column chromatography to prepare INM-A. To track alkaloids, INM-A was conducted with Dragendorff's, Mayer's, and Wagner's reagents. HPLC and UV–Vis spectrophotometer were applied to analyze the chemical profile and relative total alkaloid content in INM-A. A psoriatic mouse model induced by imiquimod (IMQ) was performed to verify in vivo efficacy of INM-A. IL-17A-dominated cellular oxygen consumption rate, oxidative stress, and cytokines in keratinocytes were measured to clarify in vitro mechanism of INM-A. Results: An alkaloid-rich fraction, INM-A, consisted of seven active alkaloid compounds 1–7 was obtained from Qing Dai. INM-A improved the skin condition severities in IMQ-induced psoriatic mice and decreased IL-17A in not only psoriatic mice but also polarized Th17 cells. In addition, INM-A targeted IL-17A to inhibit inflammation and OXPHOS-driven oxidative stress in human keratinocytes. Conclusion: Accordingly, INM-A manufactured from Qing Dai may be a promising lead phytopharmaceutical for further IL-17A-related inflammatory disease studies such as psoriasis.

Original language	English
Article number	116924
Pages (from-to)	116924
Journal	Journal of Ethnopharmacology
Volume	318
Issue number	Pt A
DOIs	https://doi.org/10.1016/j.jep.2023.116924
State	Published - 10 01 2024
Externally published	Yes

Bibliographical note

Keywords

Alkaloid-rich phytopharmaceutical (INM-A)
Anti-OXPHOS-driven oxidative stress
Anti-inflammation
IL-17A inhibition
Psoriasis
Qing Dai (Indigo Naturalis)
Keratinocytes/pathology
Imiquimod
Humans
Psoriasis/chemically induced
Antineoplastic Agents/therapeutic use
Skin/pathology
Animals
Quality of Life
Mice
Mice, Inbred BALB C
Interleukin-17
Alkaloids/adverse effects
Disease Models, Animal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.jep.2023.116924

Cite this

@article{89158c62ae794bf585c1bc6ae486a05d,

title = "An alkaloid-rich phytopharmaceutical prepared from Qing Dai against IL-17A-induced psoriasis",

abstract = "Ethnopharmacological relevance: Psoriasis is a chronic inflammatory disease due to immune dysregulation that cannot be cured. The skin conditions of psoriasis negatively impact patients{\textquoteright} quality of life worldwide. Qing Dai (Indigo Naturalis), a traditional Chinese medicine (TCM) processed from Strobilanthes cusia is a clinical medicine used for psoriasis patient in Taiwan and the gene overexpression of interleukin (IL)-17A could be notably reduced in skin lesions after using Qing Dai ointment and its alkaloid ingredients. Aim of the study: To develop a potential anti-psoriatic phytopharmaceutical, an alkaloid-rich fraction named INM-A was prepared from Qing Dai. The chemical profile and anti-psoriatic activity of INM-A were analyzed and evaluated to define its in vitro mechanism and in vivo efficacy for psoriasis therapy. Materials and methods: Dowex{\textregistered} 50WX4 hydrogen form resin was used for column chromatography to prepare INM-A. To track alkaloids, INM-A was conducted with Dragendorff's, Mayer's, and Wagner's reagents. HPLC and UV–Vis spectrophotometer were applied to analyze the chemical profile and relative total alkaloid content in INM-A. A psoriatic mouse model induced by imiquimod (IMQ) was performed to verify in vivo efficacy of INM-A. IL-17A-dominated cellular oxygen consumption rate, oxidative stress, and cytokines in keratinocytes were measured to clarify in vitro mechanism of INM-A. Results: An alkaloid-rich fraction, INM-A, consisted of seven active alkaloid compounds 1–7 was obtained from Qing Dai. INM-A improved the skin condition severities in IMQ-induced psoriatic mice and decreased IL-17A in not only psoriatic mice but also polarized Th17 cells. In addition, INM-A targeted IL-17A to inhibit inflammation and OXPHOS-driven oxidative stress in human keratinocytes. Conclusion: Accordingly, INM-A manufactured from Qing Dai may be a promising lead phytopharmaceutical for further IL-17A-related inflammatory disease studies such as psoriasis.",

keywords = "Alkaloid-rich phytopharmaceutical (INM-A), Anti-OXPHOS-driven oxidative stress, Anti-inflammation, IL-17A inhibition, Psoriasis, Qing Dai (Indigo Naturalis), Keratinocytes/pathology, Imiquimod, Humans, Psoriasis/chemically induced, Antineoplastic Agents/therapeutic use, Skin/pathology, Animals, Quality of Life, Mice, Mice, Inbred BALB C, Interleukin-17, Alkaloids/adverse effects, Disease Models, Animal",

author = "Lee, {Chia Lin} and Wang, {Chien Ming} and Song, {Ying Chyi} and Liu, {Chuan Teng} and Chu, {Mei Yun} and Yen, {Hung Rong}",

year = "2024",

month = jan,

day = "10",

doi = "10.1016/j.jep.2023.116924",

language = "英语",

volume = "318",

pages = "116924",

journal = "Journal of Ethnopharmacology",

issn = "0378-8741",

publisher = "Elsevier Ireland Ltd",

number = "Pt A",

}

TY - JOUR

T1 - An alkaloid-rich phytopharmaceutical prepared from Qing Dai against IL-17A-induced psoriasis

AU - Lee, Chia Lin

AU - Wang, Chien Ming

AU - Song, Ying Chyi

AU - Liu, Chuan Teng

AU - Chu, Mei Yun

AU - Yen, Hung Rong

PY - 2024/1/10

Y1 - 2024/1/10

N2 - Ethnopharmacological relevance: Psoriasis is a chronic inflammatory disease due to immune dysregulation that cannot be cured. The skin conditions of psoriasis negatively impact patients’ quality of life worldwide. Qing Dai (Indigo Naturalis), a traditional Chinese medicine (TCM) processed from Strobilanthes cusia is a clinical medicine used for psoriasis patient in Taiwan and the gene overexpression of interleukin (IL)-17A could be notably reduced in skin lesions after using Qing Dai ointment and its alkaloid ingredients. Aim of the study: To develop a potential anti-psoriatic phytopharmaceutical, an alkaloid-rich fraction named INM-A was prepared from Qing Dai. The chemical profile and anti-psoriatic activity of INM-A were analyzed and evaluated to define its in vitro mechanism and in vivo efficacy for psoriasis therapy. Materials and methods: Dowex® 50WX4 hydrogen form resin was used for column chromatography to prepare INM-A. To track alkaloids, INM-A was conducted with Dragendorff's, Mayer's, and Wagner's reagents. HPLC and UV–Vis spectrophotometer were applied to analyze the chemical profile and relative total alkaloid content in INM-A. A psoriatic mouse model induced by imiquimod (IMQ) was performed to verify in vivo efficacy of INM-A. IL-17A-dominated cellular oxygen consumption rate, oxidative stress, and cytokines in keratinocytes were measured to clarify in vitro mechanism of INM-A. Results: An alkaloid-rich fraction, INM-A, consisted of seven active alkaloid compounds 1–7 was obtained from Qing Dai. INM-A improved the skin condition severities in IMQ-induced psoriatic mice and decreased IL-17A in not only psoriatic mice but also polarized Th17 cells. In addition, INM-A targeted IL-17A to inhibit inflammation and OXPHOS-driven oxidative stress in human keratinocytes. Conclusion: Accordingly, INM-A manufactured from Qing Dai may be a promising lead phytopharmaceutical for further IL-17A-related inflammatory disease studies such as psoriasis.

AB - Ethnopharmacological relevance: Psoriasis is a chronic inflammatory disease due to immune dysregulation that cannot be cured. The skin conditions of psoriasis negatively impact patients’ quality of life worldwide. Qing Dai (Indigo Naturalis), a traditional Chinese medicine (TCM) processed from Strobilanthes cusia is a clinical medicine used for psoriasis patient in Taiwan and the gene overexpression of interleukin (IL)-17A could be notably reduced in skin lesions after using Qing Dai ointment and its alkaloid ingredients. Aim of the study: To develop a potential anti-psoriatic phytopharmaceutical, an alkaloid-rich fraction named INM-A was prepared from Qing Dai. The chemical profile and anti-psoriatic activity of INM-A were analyzed and evaluated to define its in vitro mechanism and in vivo efficacy for psoriasis therapy. Materials and methods: Dowex® 50WX4 hydrogen form resin was used for column chromatography to prepare INM-A. To track alkaloids, INM-A was conducted with Dragendorff's, Mayer's, and Wagner's reagents. HPLC and UV–Vis spectrophotometer were applied to analyze the chemical profile and relative total alkaloid content in INM-A. A psoriatic mouse model induced by imiquimod (IMQ) was performed to verify in vivo efficacy of INM-A. IL-17A-dominated cellular oxygen consumption rate, oxidative stress, and cytokines in keratinocytes were measured to clarify in vitro mechanism of INM-A. Results: An alkaloid-rich fraction, INM-A, consisted of seven active alkaloid compounds 1–7 was obtained from Qing Dai. INM-A improved the skin condition severities in IMQ-induced psoriatic mice and decreased IL-17A in not only psoriatic mice but also polarized Th17 cells. In addition, INM-A targeted IL-17A to inhibit inflammation and OXPHOS-driven oxidative stress in human keratinocytes. Conclusion: Accordingly, INM-A manufactured from Qing Dai may be a promising lead phytopharmaceutical for further IL-17A-related inflammatory disease studies such as psoriasis.

KW - Alkaloid-rich phytopharmaceutical (INM-A)

KW - Anti-OXPHOS-driven oxidative stress

KW - Anti-inflammation

KW - IL-17A inhibition

KW - Psoriasis

KW - Qing Dai (Indigo Naturalis)

KW - Keratinocytes/pathology

KW - Imiquimod

KW - Humans

KW - Psoriasis/chemically induced

KW - Antineoplastic Agents/therapeutic use

KW - Skin/pathology

KW - Animals

KW - Quality of Life

KW - Mice

KW - Mice, Inbred BALB C

KW - Interleukin-17

KW - Alkaloids/adverse effects

KW - Disease Models, Animal

UR - http://www.scopus.com/inward/record.url?scp=85165251510&partnerID=8YFLogxK

U2 - 10.1016/j.jep.2023.116924

DO - 10.1016/j.jep.2023.116924

M3 - 文章

C2 - 37454748

AN - SCOPUS:85165251510

SN - 0378-8741

VL - 318

SP - 116924

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

IS - Pt A

M1 - 116924

ER -

An alkaloid-rich phytopharmaceutical prepared from Qing Dai against IL-17A-induced psoriasis

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this