An aptamer-based sandwich assay for detection of alpha-defensin human neutrophil protein 1 on a microfluidic platform

Rishabh Gandotra, To Wen Chen, Feng Chih Kuo, Mel S. Lee*, Gwo Bin Lee*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

5 Scopus citations


The diagnosis of periprosthetic joint infection (PJI) remains a labor-intensive and challenging issue, with life-threatening complications associated with misdiagnoses. Superior diagnostic approaches are therefore urgently needed, and synovial biomarkers are gaining substantial attention in this capacity. A new aptamer-based sandwich assay was developed where the aptamer probes specific to one such biomarker, alpha-defensin human neutrophil protein 1 (HNP 1), was integrated herein into a new microfluidic platform. The magnetic beads coated with the primary aptamer probe were able to bind the target protein with high affinity and high specificity in synovial fluid and a fluorescent-labelled secondary aptamer were further used to quantify HNP 1 in a sandwich approach. Up to four clinical samples with low volume (∼50 μL each) in a much faster assay including detection within <60 min with 100% accuracy (with totally 13 clinical samples without the need of sample pretreatment) through the use of the aptamer-based sandwich assay were automatically detected on a single chip. The wide dynamic range of this compact device, 0.5–100 mg/L, highlights its utility for future PJI diagnostics in the clinic.

Original languageEnglish
Article number115120
Pages (from-to)115120
JournalBiosensors and Bioelectronics
StatePublished - 01 06 2023
Externally publishedYes

Bibliographical note

Copyright © 2023. Published by Elsevier B.V.


  • Aptamer
  • Human neutrophil peptide 1
  • Microfluidics
  • Paper-based assay
  • Periprosthetic joint infection
  • Synovial fluid
  • Humans
  • Neutrophils
  • Oligonucleotides
  • Synovial Fluid
  • Arthritis, Infectious/diagnosis
  • Sensitivity and Specificity
  • Biosensing Techniques
  • Biomarkers
  • alpha-Defensins


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