Abstract
This study presents an integrated microfluidic system which can identify the vancomycin resistant gene (vanA) from live hetero-bacteria automatically. In this study, a new approach to diagnose the vanA gene from live hetero-bacteria by using ethidium monoazide (EMA) and loop-mediated isothermal amplification (LAMP) was proposed and its feasibility was tested and verified. In addition, an integrated microfluidic system including a microfluidic chip and a home-made control system was also demonstrated. The experimental results showed that the developed system can detect the vanA gene from 'live' Enterococcus but not 'dead' Enterococcus successfully with a detection limit of 10 colony formation units (CFU). The entire process including the sample pre-treatment process could be automated within 1 hour. This is the first time that an integrated microfluidic system was demonstrated to diagnose the vanA gene from live bacteria by using a molecular diagnosis approach. With its high sensitivity and accuracy, the proposed system may be promising to verify antibiotic resistance genes from live hetero-bacteria which cannot be achieved by using the existing diagnostic methods.
Original language | English |
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Title of host publication | 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 |
Publisher | Institute of Electrical and Electronics Engineers Inc. |
Pages | 591-594 |
Number of pages | 4 |
ISBN (Electronic) | 9781509019472 |
DOIs | |
State | Published - 28 11 2016 |
Externally published | Yes |
Event | 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 - Sendai, Japan Duration: 17 04 2016 → 20 04 2016 |
Publication series
Name | 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 |
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Conference
Conference | 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 |
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Country/Territory | Japan |
City | Sendai |
Period | 17/04/16 → 20/04/16 |
Bibliographical note
Publisher Copyright:© 2016 IEEE.
Keywords
- live hetero-bacteria
- loop-mediated isothermal amplification
- microfluidic
- vancomycin resistant gene