Abstract
Systematic evolution of ligands by exponential enrichment (SELEX) is a complicated and time-consuming process which requires several iterative rounds to screen aptamers with high specificity and affinity. In this work, a new automatic SELEX platform was developed to select aptamers specific towards an important biomarker, human neutrophil peptide 1-3 (HNP 1-3), for diagnosis of joint infection. Promising aptamer candidates that can be targeted against HNP 1-3 were successfully selected, which could be applied as an artificial antibody.
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 749-750 |
| Number of pages | 2 |
| ISBN (Electronic) | 9781733419031 |
| State | Published - 2021 |
| Externally published | Yes |
| Event | 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States Duration: 10 10 2021 → 14 10 2021 |
Publication series
| Name | MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Conference
| Conference | 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 |
|---|---|
| Country/Territory | United States |
| City | Palm Springs, Virtual |
| Period | 10/10/21 → 14/10/21 |
Bibliographical note
Publisher Copyright:© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
Keywords
- Aptamers
- Human neutrophil peptide 1-3
- Microfluidics
- Periprosthetic joint infection
- Systematic evolution of ligands by exponential enrichment