Abstract
Diagnosis of periprosthetic joint infection (PJI) is a clinical challenge for implant surgery. In this work, a new integrated microfluidic system by using ethidium monoazide (EMA) treatment and loop-mediated isothermal amplification (LAMP) was reported for fast diagnosis of PJI. It is the first time that EMA and LAMP approaches are used for detection of "live" bacteria in joint fluids. Furthermore, this whole diagnostic process including sample pretreatment, DNA amplification and optical detection was automatically performed on the integrated microfluidic chip by using a compact custom-made system. Therefore, this detection method of PJI is potentially feasible for on-site diagnosis.
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 317-319 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| State | Published - 2015 |
| Externally published | Yes |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 25 10 2015 → 29 10 2015 |
Publication series
| Name | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Conference
| Conference | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
|---|---|
| Country/Territory | Korea, Republic of |
| City | Gyeongju |
| Period | 25/10/15 → 29/10/15 |
Bibliographical note
Publisher Copyright:© 15CBMS-0001.
Keywords
- Ethidium monoazide (EMA)
- Loop-mediated isothermal amplification (LAMP)
- Microfluidics
- Periprosthetic joint infection (PJI)