An integrated microfluidic system for rapid detection and typing of live bacteria from human joint fluidic samples

Wen Hsin Chang; Chih Hung Wang; Chih Lin Lin; Jiunn Jong Wu; Mel S. Lee; Gwo Bin Lee

doi:10.1109/NANO.2014.6968166

An integrated microfluidic system for rapid detection and typing of live bacteria from human joint fluidic samples

Wen Hsin Chang
, Chih Hung Wang
, Chih Lin Lin
, Jiunn Jong Wu
, Mel S. Lee^*
, Gwo Bin Lee

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Periprosthetic joint infection (PJI) is a devastating complication after arthroplasty. Two-stage reimplantation procedure is the most common practice to prevent the reoccurrence of PJI. However, it should be assisted by a rapid, accurate and comprehensive bacteria detection tool. In this study, a new integrated microfluidic system capable of performing sample pre-treatment, a nanogold detection to provide live bacteria information (30 minutes) and a PCR reaction (further 40 minutes) to distinguished bacteria types for antibiotic treatment automatically without human intervention is proposed. The experimental results demonstrated that this powerful tool can serve as a base for clinical decision-making in the near future.

Original language	English
Title of host publication	Proceedings of the IEEE Conference on Nanotechnology
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	752-755
Number of pages	4
ISBN (Electronic)	9781479956227
DOIs	https://doi.org/10.1109/NANO.2014.6968166
State	Published - 26 11 2014
Externally published	Yes
Event	2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014 - Toronto, Canada Duration: 18 08 2014 → 21 08 2014

Publication series

Name	Proceedings of the IEEE Conference on Nanotechnology
ISSN (Electronic)	1944-9399

Conference

Conference	2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014
Country/Territory	Canada
City	Toronto
Period	18/08/14 → 21/08/14

Bibliographical note

Publisher Copyright:
© 2014 IEEE.

Access to Document

10.1109/NANO.2014.6968166

Cite this

Chang, W. H., Wang, C. H., Lin, C. L., Wu, J. J., Lee, M. S., & Lee, G. B. (2014). An integrated microfluidic system for rapid detection and typing of live bacteria from human joint fluidic samples. In Proceedings of the IEEE Conference on Nanotechnology (pp. 752-755). Article 6968166 (Proceedings of the IEEE Conference on Nanotechnology). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NANO.2014.6968166

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title = "An integrated microfluidic system for rapid detection and typing of live bacteria from human joint fluidic samples",

abstract = "Periprosthetic joint infection (PJI) is a devastating complication after arthroplasty. Two-stage reimplantation procedure is the most common practice to prevent the reoccurrence of PJI. However, it should be assisted by a rapid, accurate and comprehensive bacteria detection tool. In this study, a new integrated microfluidic system capable of performing sample pre-treatment, a nanogold detection to provide live bacteria information (30 minutes) and a PCR reaction (further 40 minutes) to distinguished bacteria types for antibiotic treatment automatically without human intervention is proposed. The experimental results demonstrated that this powerful tool can serve as a base for clinical decision-making in the near future.",

author = "Chang, \{Wen Hsin\} and Wang, \{Chih Hung\} and Lin, \{Chih Lin\} and Wu, \{Jiunn Jong\} and Lee, \{Mel S.\} and Lee, \{Gwo Bin\}",

note = "Publisher Copyright: {\textcopyright} 2014 IEEE.; 2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014 ; Conference date: 18-08-2014 Through 21-08-2014",

year = "2014",

month = nov,

day = "26",

doi = "10.1109/NANO.2014.6968166",

language = "英语",

series = "Proceedings of the IEEE Conference on Nanotechnology",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

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Chang, WH, Wang, CH, Lin, CL, Wu, JJ, Lee, MS & Lee, GB 2014, An integrated microfluidic system for rapid detection and typing of live bacteria from human joint fluidic samples. in Proceedings of the IEEE Conference on Nanotechnology., 6968166, Proceedings of the IEEE Conference on Nanotechnology, Institute of Electrical and Electronics Engineers Inc., pp. 752-755, 2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014, Toronto, Canada, 18/08/14. https://doi.org/10.1109/NANO.2014.6968166

An integrated microfluidic system for rapid detection and typing of live bacteria from human joint fluidic samples. / Chang, Wen Hsin; Wang, Chih Hung; Lin, Chih Lin et al.
Proceedings of the IEEE Conference on Nanotechnology. Institute of Electrical and Electronics Engineers Inc., 2014. p. 752-755 6968166 (Proceedings of the IEEE Conference on Nanotechnology).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Lee, Mel S.

AU - Lee, Gwo Bin

PY - 2014/11/26

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N2 - Periprosthetic joint infection (PJI) is a devastating complication after arthroplasty. Two-stage reimplantation procedure is the most common practice to prevent the reoccurrence of PJI. However, it should be assisted by a rapid, accurate and comprehensive bacteria detection tool. In this study, a new integrated microfluidic system capable of performing sample pre-treatment, a nanogold detection to provide live bacteria information (30 minutes) and a PCR reaction (further 40 minutes) to distinguished bacteria types for antibiotic treatment automatically without human intervention is proposed. The experimental results demonstrated that this powerful tool can serve as a base for clinical decision-making in the near future.

AB - Periprosthetic joint infection (PJI) is a devastating complication after arthroplasty. Two-stage reimplantation procedure is the most common practice to prevent the reoccurrence of PJI. However, it should be assisted by a rapid, accurate and comprehensive bacteria detection tool. In this study, a new integrated microfluidic system capable of performing sample pre-treatment, a nanogold detection to provide live bacteria information (30 minutes) and a PCR reaction (further 40 minutes) to distinguished bacteria types for antibiotic treatment automatically without human intervention is proposed. The experimental results demonstrated that this powerful tool can serve as a base for clinical decision-making in the near future.

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T3 - Proceedings of the IEEE Conference on Nanotechnology

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Chang WH, Wang CH, Lin CL, Wu JJ, Lee MS, Lee GB. An integrated microfluidic system for rapid detection and typing of live bacteria from human joint fluidic samples. In Proceedings of the IEEE Conference on Nanotechnology. Institute of Electrical and Electronics Engineers Inc. 2014. p. 752-755. 6968166. (Proceedings of the IEEE Conference on Nanotechnology). doi: 10.1109/NANO.2014.6968166

An integrated microfluidic system for rapid detection and typing of live bacteria from human joint fluidic samples

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