Analysis of epigenetic regulation of hypoxia-induced epithelial–mesenchymal transition in cancer cells by quantitative chromatin immunoprecipitation of histone deacetylase 3 (HDAC3)

Jian Qiu Wang; Min Zu Wu; Kou Juey Wu

doi:10.1007/978-1-4939-3667-0_3

Analysis of epigenetic regulation of hypoxia-induced epithelial–mesenchymal transition in cancer cells by quantitative chromatin immunoprecipitation of histone deacetylase 3 (HDAC3)

Jian Qiu Wang
, Min Zu Wu
, Kou Juey Wu^*

^*Corresponding author for this work

Hangzhou Normal University
Salk Institute for Biological Studies
China Medical University Taichung

Research output: Contribution to journal › Journal Article › peer-review

4 Scopus citations

Abstract

Epigenetics plays a key role in gene expression control. Histone modifications including acetylation/ deacetylation or methylation/demethylation are major epigenetic mechanisms known to regulate epithelial–mesenchymal transition (EMT)-associated gene expression during hypoxia-induced cancer metastasis. Chromatin immunoprecipitation (ChIP) assay is a powerful tool for investigation of histone modification patterns of genes of interest. In this chapter, we describe a protocol that uses chromatin immunoprecipitation (ChIP) to analyze the epigenetic regulation of EMT marker genes by deacetylation of acetylated Histone 3 Lys 4 (H3K4Ac) under hypoxia in a head and neck cancer cell line FaDu cells. Not only a method of ChIP coupled by real-time quantitative PCR but also the detailed conditions are provided based on our previously published studies.

Original language	English
Pages (from-to)	23-29
Number of pages	7
Journal	Methods in Molecular Biology
Volume	1436
DOIs	https://doi.org/10.1007/978-1-4939-3667-0_3
State	Published - 2016
Externally published	Yes

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2016.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Chromatin immunoprecipitation
Epithelial–mesenchymal transition
HDAC3
Histone modification
Hypoxia
Real-time qPCR

Access to Document

10.1007/978-1-4939-3667-0_3

Cite this

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title = "Analysis of epigenetic regulation of hypoxia-induced epithelial–mesenchymal transition in cancer cells by quantitative chromatin immunoprecipitation of histone deacetylase 3 (HDAC3)",

abstract = "Epigenetics plays a key role in gene expression control. Histone modifications including acetylation/ deacetylation or methylation/demethylation are major epigenetic mechanisms known to regulate epithelial–mesenchymal transition (EMT)-associated gene expression during hypoxia-induced cancer metastasis. Chromatin immunoprecipitation (ChIP) assay is a powerful tool for investigation of histone modification patterns of genes of interest. In this chapter, we describe a protocol that uses chromatin immunoprecipitation (ChIP) to analyze the epigenetic regulation of EMT marker genes by deacetylation of acetylated Histone 3 Lys 4 (H3K4Ac) under hypoxia in a head and neck cancer cell line FaDu cells. Not only a method of ChIP coupled by real-time quantitative PCR but also the detailed conditions are provided based on our previously published studies.",

keywords = "Chromatin immunoprecipitation, Epithelial–mesenchymal transition, HDAC3, Histone modification, Hypoxia, Real-time qPCR",

author = "Wang, \{Jian Qiu\} and Wu, \{Min Zu\} and Wu, \{Kou Juey\}",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",

year = "2016",

doi = "10.1007/978-1-4939-3667-0\_3",

language = "英语",

volume = "1436",

pages = "23--29",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

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T1 - Analysis of epigenetic regulation of hypoxia-induced epithelial–mesenchymal transition in cancer cells by quantitative chromatin immunoprecipitation of histone deacetylase 3 (HDAC3)

AU - Wang, Jian Qiu

AU - Wu, Min Zu

AU - Wu, Kou Juey

N1 - Publisher Copyright: © Springer Science+Business Media New York 2016.

PY - 2016

Y1 - 2016

N2 - Epigenetics plays a key role in gene expression control. Histone modifications including acetylation/ deacetylation or methylation/demethylation are major epigenetic mechanisms known to regulate epithelial–mesenchymal transition (EMT)-associated gene expression during hypoxia-induced cancer metastasis. Chromatin immunoprecipitation (ChIP) assay is a powerful tool for investigation of histone modification patterns of genes of interest. In this chapter, we describe a protocol that uses chromatin immunoprecipitation (ChIP) to analyze the epigenetic regulation of EMT marker genes by deacetylation of acetylated Histone 3 Lys 4 (H3K4Ac) under hypoxia in a head and neck cancer cell line FaDu cells. Not only a method of ChIP coupled by real-time quantitative PCR but also the detailed conditions are provided based on our previously published studies.

AB - Epigenetics plays a key role in gene expression control. Histone modifications including acetylation/ deacetylation or methylation/demethylation are major epigenetic mechanisms known to regulate epithelial–mesenchymal transition (EMT)-associated gene expression during hypoxia-induced cancer metastasis. Chromatin immunoprecipitation (ChIP) assay is a powerful tool for investigation of histone modification patterns of genes of interest. In this chapter, we describe a protocol that uses chromatin immunoprecipitation (ChIP) to analyze the epigenetic regulation of EMT marker genes by deacetylation of acetylated Histone 3 Lys 4 (H3K4Ac) under hypoxia in a head and neck cancer cell line FaDu cells. Not only a method of ChIP coupled by real-time quantitative PCR but also the detailed conditions are provided based on our previously published studies.

KW - Chromatin immunoprecipitation

KW - Epithelial–mesenchymal transition

KW - HDAC3

KW - Histone modification

KW - Hypoxia

KW - Real-time qPCR

UR - https://www.scopus.com/pages/publications/85007565951

U2 - 10.1007/978-1-4939-3667-0_3

DO - 10.1007/978-1-4939-3667-0_3

M3 - 文章

C2 - 27246206

AN - SCOPUS:85007565951

SN - 1064-3745

VL - 1436

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Analysis of epigenetic regulation of hypoxia-induced epithelial–mesenchymal transition in cancer cells by quantitative chromatin immunoprecipitation of histone deacetylase 3 (HDAC3)

Abstract

Bibliographical note

UN SDGs

Keywords

Access to Document

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