Analysis on the status of hepatitis B virus DNA in peripheral blood mononuclear cells of chronic hepatitis B patients

F. ‐H WONG; Y. ‐F LIAW; C. HU; Y. CHAO; C. CHANG; C. C. PAO

doi:10.1111/j.1440-1746.1993.tb01687.x

Analysis on the status of hepatitis B virus DNA in peripheral blood mononuclear cells of chronic hepatitis B patients

F. ‐H WONG, Y. ‐F LIAW, C. HU, Y. CHAO, C. CHANG^*, C. C. PAO

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

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Abstract

Peripheral blood mononuclear cells (PBMC) from 46 hepatitis B virus (HBV) surface antigen (HBsAg)‐positive chronic active hepatitis (CAH) patients were examined for the presence and for the physical states of HBV DNA by Southern blot analysis. Twenty‐one of 46 patients (45.7%) were found to have HBV DNA in their PBMC. Further fractionation of PBMC of these patients indicated that HBV DNA can be found in both T and B lymphocytes but not in macrophages. Results also suggested that the PBMC‐associated HBV DNA was in a changing and unstable state. No HBV DNA could be detected in the long‐term culture of either Epstein‐Barr virus‐immortalized B cells or in interleukin‐2‐cultured T cells of these patients.

Original language	English
Pages (from-to)	S75-S79
Journal	Journal of Gastroenterology and Hepatology (Australia)
Volume	8
Issue number	1 S
DOIs	https://doi.org/10.1111/j.1440-1746.1993.tb01687.x
State	Published - 01 1993
Externally published	Yes

Keywords

HBV DNA
Key words
chronic active hepatitis
peripheral blood mononuclear cells.

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1440-1746.1993.tb01687.x

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title = "Analysis on the status of hepatitis B virus DNA in peripheral blood mononuclear cells of chronic hepatitis B patients",

abstract = "Peripheral blood mononuclear cells (PBMC) from 46 hepatitis B virus (HBV) surface antigen (HBsAg)‐positive chronic active hepatitis (CAH) patients were examined for the presence and for the physical states of HBV DNA by Southern blot analysis. Twenty‐one of 46 patients (45.7%) were found to have HBV DNA in their PBMC. Further fractionation of PBMC of these patients indicated that HBV DNA can be found in both T and B lymphocytes but not in macrophages. Results also suggested that the PBMC‐associated HBV DNA was in a changing and unstable state. No HBV DNA could be detected in the long‐term culture of either Epstein‐Barr virus‐immortalized B cells or in interleukin‐2‐cultured T cells of these patients.",

keywords = "HBV DNA, Key words, chronic active hepatitis, peripheral blood mononuclear cells.",

author = "WONG, {F. ‐H} and LIAW, {Y. ‐F} and C. HU and Y. CHAO and C. CHANG and PAO, {C. C.}",

year = "1993",

month = jan,

doi = "10.1111/j.1440-1746.1993.tb01687.x",

language = "英语",

volume = "8",

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issn = "0815-9319",

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TY - JOUR

T1 - Analysis on the status of hepatitis B virus DNA in peripheral blood mononuclear cells of chronic hepatitis B patients

AU - WONG, F. ‐H

AU - LIAW, Y. ‐F

AU - HU, C.

AU - CHAO, Y.

AU - CHANG, C.

AU - PAO, C. C.

PY - 1993/1

Y1 - 1993/1

N2 - Peripheral blood mononuclear cells (PBMC) from 46 hepatitis B virus (HBV) surface antigen (HBsAg)‐positive chronic active hepatitis (CAH) patients were examined for the presence and for the physical states of HBV DNA by Southern blot analysis. Twenty‐one of 46 patients (45.7%) were found to have HBV DNA in their PBMC. Further fractionation of PBMC of these patients indicated that HBV DNA can be found in both T and B lymphocytes but not in macrophages. Results also suggested that the PBMC‐associated HBV DNA was in a changing and unstable state. No HBV DNA could be detected in the long‐term culture of either Epstein‐Barr virus‐immortalized B cells or in interleukin‐2‐cultured T cells of these patients.

AB - Peripheral blood mononuclear cells (PBMC) from 46 hepatitis B virus (HBV) surface antigen (HBsAg)‐positive chronic active hepatitis (CAH) patients were examined for the presence and for the physical states of HBV DNA by Southern blot analysis. Twenty‐one of 46 patients (45.7%) were found to have HBV DNA in their PBMC. Further fractionation of PBMC of these patients indicated that HBV DNA can be found in both T and B lymphocytes but not in macrophages. Results also suggested that the PBMC‐associated HBV DNA was in a changing and unstable state. No HBV DNA could be detected in the long‐term culture of either Epstein‐Barr virus‐immortalized B cells or in interleukin‐2‐cultured T cells of these patients.

KW - HBV DNA

KW - Key words

KW - chronic active hepatitis

KW - peripheral blood mononuclear cells.

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U2 - 10.1111/j.1440-1746.1993.tb01687.x

DO - 10.1111/j.1440-1746.1993.tb01687.x

M3 - 文章

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JF - Journal of Gastroenterology and Hepatology (Australia)

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Analysis on the status of hepatitis B virus DNA in peripheral blood mononuclear cells of chronic hepatitis B patients

Abstract

Keywords

UN SDGs

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