TY - JOUR
T1 - Angelica dahurica attenuates melanogenesis in B16F0 cells by repressing Wnt/β-catenin signaling
AU - Fang, Chien Liang
AU - Goswami, Debakshee
AU - Kuo, Chia Hua
AU - Day, Cecilia Hsuan
AU - Lin, Mei Yi
AU - Ho, Tsung Jung
AU - Yang, Liang Yo
AU - Hsieh, Dennis Jine Yuan
AU - Lin, Tzu Kai
AU - Huang, Chih Yang
N1 - Publisher Copyright:
© 2022, The Author(s) under exclusive licence to The Korean Society of Toxicogenomics and Toxicoproteomics.
PY - 2023/1
Y1 - 2023/1
N2 - Background: Melanogenesis is a complex process which is tightly regulated by several enzymes. However, abnormal melanogenesis can cause severe dermatological problems. Roots of Angelica dahurica have been used for skin care as a part of traditional Chinese medicine for many generations. However, the role of A. dahurica in melanogenesis remains unclear. Objective: Previous in vitro and in vivo studies have demonstrated that NK-1R exerts positive effects in melanogenesis via the Wnt/βcatenin signaling pathway. In this study, we investigated the effects of A. dahurica ethanol extract (ADE) on NK-1R and Wnt/β-catenin signaling, and evaluated the effect of NK-1R on melanogenesis in B16F0 cells. Results: Angelica dahurica ethanol extract efficiently downregulated Neurokinin-1 receptor and Wnt/β-catenin signaling by decreasing the expression of β-catenin, MITF, LEF-1, TYR, TRP1, and TRP2 and increasing the expression of GSK3β, which resulted from the weakened expression of the Neurokinin-1 receptor inhibitor [Sar9,Met(O2)11]-Substance P (SMSP). Furthermore, the intracellular melanin assay and cellular tyrosinase activity confirmed these findings. Conclusion: This study suggests that ADE has potential to downregulate Neurokinin-1 receptor in SMSP-induced B16F0 cells, thereby repressing the Wnt/β-catenin signaling and reduces melanin production. Graphical abstract: [Figure not available: see fulltext.]
AB - Background: Melanogenesis is a complex process which is tightly regulated by several enzymes. However, abnormal melanogenesis can cause severe dermatological problems. Roots of Angelica dahurica have been used for skin care as a part of traditional Chinese medicine for many generations. However, the role of A. dahurica in melanogenesis remains unclear. Objective: Previous in vitro and in vivo studies have demonstrated that NK-1R exerts positive effects in melanogenesis via the Wnt/βcatenin signaling pathway. In this study, we investigated the effects of A. dahurica ethanol extract (ADE) on NK-1R and Wnt/β-catenin signaling, and evaluated the effect of NK-1R on melanogenesis in B16F0 cells. Results: Angelica dahurica ethanol extract efficiently downregulated Neurokinin-1 receptor and Wnt/β-catenin signaling by decreasing the expression of β-catenin, MITF, LEF-1, TYR, TRP1, and TRP2 and increasing the expression of GSK3β, which resulted from the weakened expression of the Neurokinin-1 receptor inhibitor [Sar9,Met(O2)11]-Substance P (SMSP). Furthermore, the intracellular melanin assay and cellular tyrosinase activity confirmed these findings. Conclusion: This study suggests that ADE has potential to downregulate Neurokinin-1 receptor in SMSP-induced B16F0 cells, thereby repressing the Wnt/β-catenin signaling and reduces melanin production. Graphical abstract: [Figure not available: see fulltext.]
KW - Angelica dahurica ethanol extract
KW - Melanogenesis
KW - Neurokinin-1 receptor
KW - Wnt/β-catenin signaling
KW - [Sar9,Met(O]-Substance P
UR - http://www.scopus.com/inward/record.url?scp=85132795632&partnerID=8YFLogxK
U2 - 10.1007/s13273-022-00250-0
DO - 10.1007/s13273-022-00250-0
M3 - 文章
AN - SCOPUS:85132795632
SN - 1738-642X
VL - 19
SP - 135
EP - 143
JO - Molecular and Cellular Toxicology
JF - Molecular and Cellular Toxicology
IS - 1
ER -
