Anti-allergic, anti-inflammatory and anti-hyperglycemic activity of chasmanthe aethiopica leaf extract and its profiling using lc/ms and glc/ms

Iriny M. Ayoub; Michal Korinek; Mohamed El-Shazly; Bernhard Wetterauer; Hesham A. El-Beshbishy; Tsong Long Hwang; Bing Hung Chen; Fang Rong Chang; Michael Wink; Abdel Nasser B. Singab; Fadia S. Youssef

doi:10.3390/plants10061118

Anti-allergic, anti-inflammatory and anti-hyperglycemic activity of chasmanthe aethiopica leaf extract and its profiling using lc/ms and glc/ms

Iriny M. Ayoub, Michal Korinek, Mohamed El-Shazly, Bernhard Wetterauer, Hesham A. El-Beshbishy, Tsong Long Hwang, Bing Hung Chen, Fang Rong Chang^*, Michael Wink^*, Abdel Nasser B. Singab^*, Fadia S. Youssef

^*Corresponding author for this work

School of Traditional Chinese Medicine

Research output: Contribution to journal › Journal Article › peer-review

38 Scopus citations

Abstract

This study aims to comprehensively explore the phytoconstituents as well as investigate the different biological activities of Chasmanthe aethiopica (Iridaceae) for the first time. Metabolic profiling of the leaf methanol extract of C. aethiopica (CAL) was carried out using HPLC-PDA-ESI-MS/MS. Twenty-nine compounds were annotated belonging to various phytochemical classes in-cluding organic acids, cinnamic acid derivatives, flavonoids, isoflavonoids, and fatty acids. Myrice-tin-3-O-rhamnoside was the major compound identified. GLC/MS analysis of the n-hexane fraction (CAL-A) resulted in the identification of 45 compounds with palmitic acid (16.08%) and methyl hexadecanoic acid ester (11.91%) representing the major constituents. CAL-A exhibited a potent anti-allergic activity as evidenced by its potent inhibition of β-hexosaminidase release triggered by A23187 and IgE by 72.7% and 48.7%, respectively. Results were comparable to that of dexame-thasone (10 nM) in the A23187 degranulation assay showing 80.7% inhibition for β-hexosaminidase release. Both the n-hexane (CAL-A) and dichloromethane (CAL-B) fractions exhibited potent anti-inflammatory activity manifested by the significant inhibition of superoxide anion generation and prohibition of elastase release. CAL showed anti-hyperglycemic activity in vivo using streptozoto-cin-induced diabetic rat model by reducing fasting blood glucose levels (FBG) by 53.44% as com-pared with STZ-treated rats along with a substantial increase in serum insulin by 22.22%. Molecular modeling studies indicated that dicaffeoylquinic acid showed the highest fitting with free binding energies (∆G) of −47.24 and −60.50 Kcal/mol for human α-amylase and α-glucosidase, respectively confirming its anti-hyperglycemic activity. Thus, C. aethiopica leaf extract could serve as an effective antioxidant natural remedy combating inflammation, allergy, and hyperglycemia.

Original language	English
Article number	1118
Journal	Plants
Volume	10
Issue number	6
DOIs	https://doi.org/10.3390/plants10061118
State	Published - 06 2021

Bibliographical note

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Anti-allergic
Anti-inflammatory
Antihyperglycemic
Chasmanthe aethiopica
GC/MS
LC/MS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/plants10061118

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Ayoub, I. M., Korinek, M., El-Shazly, M., Wetterauer, B., El-Beshbishy, H. A., Hwang, T. L., Chen, B. H., Chang, F. R., Wink, M., Singab, A. N. B., & Youssef, F. S. (2021). Anti-allergic, anti-inflammatory and anti-hyperglycemic activity of chasmanthe aethiopica leaf extract and its profiling using lc/ms and glc/ms. Plants, 10(6), Article 1118. https://doi.org/10.3390/plants10061118

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title = "Anti-allergic, anti-inflammatory and anti-hyperglycemic activity of chasmanthe aethiopica leaf extract and its profiling using lc/ms and glc/ms",

abstract = "This study aims to comprehensively explore the phytoconstituents as well as investigate the different biological activities of Chasmanthe aethiopica (Iridaceae) for the first time. Metabolic profiling of the leaf methanol extract of C. aethiopica (CAL) was carried out using HPLC-PDA-ESI-MS/MS. Twenty-nine compounds were annotated belonging to various phytochemical classes in-cluding organic acids, cinnamic acid derivatives, flavonoids, isoflavonoids, and fatty acids. Myrice-tin-3-O-rhamnoside was the major compound identified. GLC/MS analysis of the n-hexane fraction (CAL-A) resulted in the identification of 45 compounds with palmitic acid (16.08%) and methyl hexadecanoic acid ester (11.91%) representing the major constituents. CAL-A exhibited a potent anti-allergic activity as evidenced by its potent inhibition of β-hexosaminidase release triggered by A23187 and IgE by 72.7% and 48.7%, respectively. Results were comparable to that of dexame-thasone (10 nM) in the A23187 degranulation assay showing 80.7% inhibition for β-hexosaminidase release. Both the n-hexane (CAL-A) and dichloromethane (CAL-B) fractions exhibited potent anti-inflammatory activity manifested by the significant inhibition of superoxide anion generation and prohibition of elastase release. CAL showed anti-hyperglycemic activity in vivo using streptozoto-cin-induced diabetic rat model by reducing fasting blood glucose levels (FBG) by 53.44% as com-pared with STZ-treated rats along with a substantial increase in serum insulin by 22.22%. Molecular modeling studies indicated that dicaffeoylquinic acid showed the highest fitting with free binding energies (∆G) of −47.24 and −60.50 Kcal/mol for human α-amylase and α-glucosidase, respectively confirming its anti-hyperglycemic activity. Thus, C. aethiopica leaf extract could serve as an effective antioxidant natural remedy combating inflammation, allergy, and hyperglycemia.",

keywords = "Anti-allergic, Anti-inflammatory, Antihyperglycemic, Chasmanthe aethiopica, GC/MS, LC/MS",

author = "Ayoub, {Iriny M.} and Michal Korinek and Mohamed El-Shazly and Bernhard Wetterauer and El-Beshbishy, {Hesham A.} and Hwang, {Tsong Long} and Chen, {Bing Hung} and Chang, {Fang Rong} and Michael Wink and Singab, {Abdel Nasser B.} and Youssef, {Fadia S.}",

note = "Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = jun,

doi = "10.3390/plants10061118",

language = "英语",

volume = "10",

journal = "Plants",

issn = "2223-7747",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "6",

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T1 - Anti-allergic, anti-inflammatory and anti-hyperglycemic activity of chasmanthe aethiopica leaf extract and its profiling using lc/ms and glc/ms

AU - Ayoub, Iriny M.

AU - Korinek, Michal

AU - El-Shazly, Mohamed

AU - Wetterauer, Bernhard

AU - El-Beshbishy, Hesham A.

AU - Hwang, Tsong Long

AU - Chen, Bing Hung

AU - Chang, Fang Rong

AU - Wink, Michael

AU - Singab, Abdel Nasser B.

AU - Youssef, Fadia S.

PY - 2021/6

Y1 - 2021/6

N2 - This study aims to comprehensively explore the phytoconstituents as well as investigate the different biological activities of Chasmanthe aethiopica (Iridaceae) for the first time. Metabolic profiling of the leaf methanol extract of C. aethiopica (CAL) was carried out using HPLC-PDA-ESI-MS/MS. Twenty-nine compounds were annotated belonging to various phytochemical classes in-cluding organic acids, cinnamic acid derivatives, flavonoids, isoflavonoids, and fatty acids. Myrice-tin-3-O-rhamnoside was the major compound identified. GLC/MS analysis of the n-hexane fraction (CAL-A) resulted in the identification of 45 compounds with palmitic acid (16.08%) and methyl hexadecanoic acid ester (11.91%) representing the major constituents. CAL-A exhibited a potent anti-allergic activity as evidenced by its potent inhibition of β-hexosaminidase release triggered by A23187 and IgE by 72.7% and 48.7%, respectively. Results were comparable to that of dexame-thasone (10 nM) in the A23187 degranulation assay showing 80.7% inhibition for β-hexosaminidase release. Both the n-hexane (CAL-A) and dichloromethane (CAL-B) fractions exhibited potent anti-inflammatory activity manifested by the significant inhibition of superoxide anion generation and prohibition of elastase release. CAL showed anti-hyperglycemic activity in vivo using streptozoto-cin-induced diabetic rat model by reducing fasting blood glucose levels (FBG) by 53.44% as com-pared with STZ-treated rats along with a substantial increase in serum insulin by 22.22%. Molecular modeling studies indicated that dicaffeoylquinic acid showed the highest fitting with free binding energies (∆G) of −47.24 and −60.50 Kcal/mol for human α-amylase and α-glucosidase, respectively confirming its anti-hyperglycemic activity. Thus, C. aethiopica leaf extract could serve as an effective antioxidant natural remedy combating inflammation, allergy, and hyperglycemia.

AB - This study aims to comprehensively explore the phytoconstituents as well as investigate the different biological activities of Chasmanthe aethiopica (Iridaceae) for the first time. Metabolic profiling of the leaf methanol extract of C. aethiopica (CAL) was carried out using HPLC-PDA-ESI-MS/MS. Twenty-nine compounds were annotated belonging to various phytochemical classes in-cluding organic acids, cinnamic acid derivatives, flavonoids, isoflavonoids, and fatty acids. Myrice-tin-3-O-rhamnoside was the major compound identified. GLC/MS analysis of the n-hexane fraction (CAL-A) resulted in the identification of 45 compounds with palmitic acid (16.08%) and methyl hexadecanoic acid ester (11.91%) representing the major constituents. CAL-A exhibited a potent anti-allergic activity as evidenced by its potent inhibition of β-hexosaminidase release triggered by A23187 and IgE by 72.7% and 48.7%, respectively. Results were comparable to that of dexame-thasone (10 nM) in the A23187 degranulation assay showing 80.7% inhibition for β-hexosaminidase release. Both the n-hexane (CAL-A) and dichloromethane (CAL-B) fractions exhibited potent anti-inflammatory activity manifested by the significant inhibition of superoxide anion generation and prohibition of elastase release. CAL showed anti-hyperglycemic activity in vivo using streptozoto-cin-induced diabetic rat model by reducing fasting blood glucose levels (FBG) by 53.44% as com-pared with STZ-treated rats along with a substantial increase in serum insulin by 22.22%. Molecular modeling studies indicated that dicaffeoylquinic acid showed the highest fitting with free binding energies (∆G) of −47.24 and −60.50 Kcal/mol for human α-amylase and α-glucosidase, respectively confirming its anti-hyperglycemic activity. Thus, C. aethiopica leaf extract could serve as an effective antioxidant natural remedy combating inflammation, allergy, and hyperglycemia.

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KW - Anti-inflammatory

KW - Antihyperglycemic

KW - Chasmanthe aethiopica

KW - GC/MS

KW - LC/MS

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U2 - 10.3390/plants10061118

DO - 10.3390/plants10061118

M3 - 文章

AN - SCOPUS:85106875693

SN - 2223-7747

VL - 10

JO - Plants

JF - Plants

IS - 6

M1 - 1118

ER -

Anti-allergic, anti-inflammatory and anti-hyperglycemic activity of chasmanthe aethiopica leaf extract and its profiling using lc/ms and glc/ms

Abstract

Bibliographical note

Keywords

UN SDGs

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