Anti-angiogenic effect of hexahydrocurcumin in rat corneal neovascularization

Chien Neng Kuo, Ching Hsein Chen, San Ni Chen, Jou Cheng Huang, Li Ju Lai, Chien Hsiung Lai, Chia Hui Hung, Chien Hsing Lee*, Chung Yi Chen

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

10 Scopus citations


Aim: This study was to investigate the anti-angiogenic effect of hexahydrocurcumin (HHC) to evaluate gene (p-basic fibroblast growth factor (bFGF)-SAINT-18 & p-vascular endothelial growth factor (VEGF)-SAINT-18 complex)-induced corneal neovascularization (CorNV) in rats. Methods: CorNV was induced in 24 eyes of 24 rats. Four groups (Group A: 0 μg, B: 0.01 μg, C: 0.1 μg, and D: 1 μg) of HHC were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus at temporal side. The 1 μg of p-bFGF-SAINT-18 & p-VEGF-SAINT-18 complex were prepared and implanted into the rat corneal stroma 1.5 mm from the limbus at the same side. Inhibition of CorNV was observed and quantified from day 1 to day 60. bFGF and VEGF protein expression were analyzed by biomicroscopic examination, western blot analysis, and immunohistochemistry. Results: Subconjunctival injection by 1 μg HHC successfully inhibited gene-induced CorNV in rats. bFGF and VEGF protein expression were reduced after 6 days. Meanwhile, the reduction of HLA-DR expression was detected. Conclusions: Our study showed that the HHC might provide an important anti-angiogenesis factor to inhibit CorNV development at the corneal experimental angiogenesis model.

Original languageEnglish
Pages (from-to)747-756
Number of pages10
JournalInternational Ophthalmology
Issue number2
StatePublished - 01 04 2018

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media Dordrecht.


  • Angiogenesis
  • Corneal neovascularization
  • Hexahydrocurcumin
  • VEGF
  • bFGF


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