Anti-Inflammatory Effects Induced by Near-Infrared Light Irradiation through M2 Macrophage Polarization

Wei Ting Liao, Chih Hsing Hung, Shih Shin Liang, Sebastian Yu, Jian He Lu, Chih Hung Lee, Chee Yin Chai, Hsin Su Yu*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

11 Scopus citations

Abstract

Near-infrared (NIR) can penetrate the dermis. NIR is able to regulate cutaneous component cells and immune cells and shows significant anti-inflammatory therapeutic effects. However, the mechanisms of these effects are largely unknown. The purpose of this study is to elucidate NIR-induced molecular mechanisms on macrophages because macrophages play initial roles in directing immune responses by their M1 or M2 polarizations. Proteomic analysis revealed that NIR radiation enhanced the expression of mitochondrial respiratory gene citrate synthase. This increased citrate synthase expression was triggered by NIR-induced H3K4 hypermethylation on the citrate synthase gene promoter but not by heat, which led to macrophage M2 polarization and finally resulted in TGFβ1 release from CD4+ cells. These cellular effects were validated in human primary macrophages and abdominal NIR-irradiated mouse experiments. In a phorbol 12-myristate 13-acetate‒induced inflammatory model on mouse ear, we confirmed that NIR irradiation induced significant anti-inflammatory effects through decreased M1 counts, reduced TNF-α, and increased CCL22 and/or TGFβ1 levels.

Original languageEnglish
Pages (from-to)2056-2066.e10
JournalJournal of Investigative Dermatology
Volume141
Issue number8
DOIs
StatePublished - 08 2021
Externally publishedYes

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