TY - JOUR
T1 - Antimicrobial, antioxidant and anticancer properties of bioactive bacteriocins produced by Lactococcus hircilactis CH4 and Lactobacillus delbrueckii GRIPUMSK isolated from homemade fermented dairy products
AU - Srinivash, Moovendran
AU - Krishnamoorthi, Raman
AU - Mahalingam, Pambayan Ulagan
AU - Malaikozhundan, Balasubramanian
AU - Kaviyadharshini, Muthuramalingam
AU - Keerthivasan, Mohan
AU - Rajkannan, Pitchaimuthu
AU - Samy, Kasi Karuppa
AU - Gurushankar, Krishnamoorthy
AU - Chung, Yu Kuo
AU - Fang, Jia You
AU - Mohandoss, Sonaimuthu
AU - Vijaykumar, Sekar
N1 - Publisher Copyright:
© 2025 Elsevier Ltd
PY - 2025/12
Y1 - 2025/12
N2 - Lactic acid bacteria (LAB) are known to produce bacteriocins. In this work, two lactic acid bacterial strains, Lactococcus hircilactis CH4 and Lactobacillus delbrueckii GRIPUMSK were isolated from fermented dairy products. They were tested for their antimicrobial, antioxidant, and anticancer properties. This research aimed to isolate bioactive bacteriocins from these two LAB strains under various conditions and thoroughly examined their molecular structure and physico-chemical properties. The optimal culture conditions for bacteriocin production such as time, temperature, pH, and carbon and nitrogen source supplementation were analyzed. The molecular weight of bacteriocins from Lb. delbrueckii GRIPUMSK and Lc. hircilactis CH4 were detected with the size of 23.8 and 8.9 kDa, respectively. The Fourier transform infrared (FTIR) spectrum of both the bacteriocins from LAB showed, –OH/N-H stretching vibrations, amide II groups and C-N stretch respectively. Proton nuclear magnetic resonance (1H NMR) spectrum of both the bacteriocins from LAB revealed seven and nine amino acids respectively. The extracted bacteriocins were revealing that bacteriocins from both strains exhibit heat resistance and stability across a wide range of detergents, organic solvents, and pH levels compared to nisin. Furthermore, we discovered that these bacteriocins effectively inhibited (89.00 ± 1.12 % and 86.00 ± 0.67 %) Helicobacter pylori biofilm formation at 100 μg/mL, demonstrated antioxidant capabilities, and showed enhanced selective cytotoxicity with IC50 at 29.87 and 42.52 μg/ml against human colon cancer cells (HT-29). In conclusion, the two isolated bacteriocins serve as safe postbiotic materials in the food and pharmaceutical industries, offering potential benefits to treat cancer and its associated bacterial infections synergistically.
AB - Lactic acid bacteria (LAB) are known to produce bacteriocins. In this work, two lactic acid bacterial strains, Lactococcus hircilactis CH4 and Lactobacillus delbrueckii GRIPUMSK were isolated from fermented dairy products. They were tested for their antimicrobial, antioxidant, and anticancer properties. This research aimed to isolate bioactive bacteriocins from these two LAB strains under various conditions and thoroughly examined their molecular structure and physico-chemical properties. The optimal culture conditions for bacteriocin production such as time, temperature, pH, and carbon and nitrogen source supplementation were analyzed. The molecular weight of bacteriocins from Lb. delbrueckii GRIPUMSK and Lc. hircilactis CH4 were detected with the size of 23.8 and 8.9 kDa, respectively. The Fourier transform infrared (FTIR) spectrum of both the bacteriocins from LAB showed, –OH/N-H stretching vibrations, amide II groups and C-N stretch respectively. Proton nuclear magnetic resonance (1H NMR) spectrum of both the bacteriocins from LAB revealed seven and nine amino acids respectively. The extracted bacteriocins were revealing that bacteriocins from both strains exhibit heat resistance and stability across a wide range of detergents, organic solvents, and pH levels compared to nisin. Furthermore, we discovered that these bacteriocins effectively inhibited (89.00 ± 1.12 % and 86.00 ± 0.67 %) Helicobacter pylori biofilm formation at 100 μg/mL, demonstrated antioxidant capabilities, and showed enhanced selective cytotoxicity with IC50 at 29.87 and 42.52 μg/ml against human colon cancer cells (HT-29). In conclusion, the two isolated bacteriocins serve as safe postbiotic materials in the food and pharmaceutical industries, offering potential benefits to treat cancer and its associated bacterial infections synergistically.
KW - Antibiofilm
KW - Bacteriocin
KW - Colon cancer
KW - Lactic acid bacteria
KW - Oxidative stress
UR - https://www.scopus.com/pages/publications/105013982581
U2 - 10.1016/j.idairyj.2025.106395
DO - 10.1016/j.idairyj.2025.106395
M3 - 文章
AN - SCOPUS:105013982581
SN - 0958-6946
VL - 171
JO - International Dairy Journal
JF - International Dairy Journal
M1 - 106395
ER -
