Antitumor progression potential of morusin suppressing STAT3 and NFκB in human hepatoma SK-Hep1 cells

Wea Lung Lin, Deng Yu Lai, Yean Jang Lee*, Nai Fang Chen, Tsui Hwa Tseng

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

46 Scopus citations

Abstract

Morusin is a prenylated flavonoid that has been isolated from the root bark of the mulberry tree (Morus species, Moraceae), a Chinese traditional medicine. It has been synthesized by our laboratory from commercially available phloroglucinol, and has demonstrated to possess antitumor effects of cell lines including A549, MCF-7, and MDA-MB-231. In this study, at non-cytotoxic concentrations, morusin altered invasive morphology and suppressed cell-matrix adhesion, cell motility and cell invasion in SK-Hep1 cells. Morusin also increased the expression of E-cadherin, an epithelial cell junction protein, decreased the expression of vimentin, a mesecnchymal marker, and α2-, α6-, β1- integrin, which regulated cancer attachment and migration. In addition, morusin reduced the activity of matrix metalloproteinase-2 and 9 (MMP-2 and MMP-9), which were involved in extracellular matrix (ECM) degradation and promoting cancer cell invasion. Furthermore, morusin suppressed the signal transducer and activator of transcription 3 (STAT3) and nuclear factor-κB (NFκB) signaling pathways, which modulate the protein expression involved in the invasion process. Finally, morusin decreased the lung colonization of the SK-Hep1 cells in the nude mice. These results indicate morusin possesses antitumor progression potential through suppressing STAT3 and NFκB.

Original languageEnglish
Pages (from-to)490-498
Number of pages9
JournalToxicology Letters
Volume232
Issue number2
DOIs
StatePublished - 02 01 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2014 Elsevier Ireland Ltd.

Keywords

  • Hepatoma
  • Morusin
  • NFκB
  • SK-Hep 1 cells
  • STAT3
  • Tumor progression

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