Apoptosis is induced in aging SV40 T antigen-transformed human fibroblasts through p53- and p21(CIP1/WAF1)-independent pathways

Yeou Ping Tsao; Shu Fang Li; Jiang Chuan Liu; Show Li Chen

doi:10.1016/S0304-3835(98)00212-2

Apoptosis is induced in aging SV40 T antigen-transformed human fibroblasts through p53- and p21(CIP1/WAF1)-independent pathways

Yeou Ping Tsao^*, Shu Fang Li, Jiang Chuan Liu, Show Li Chen

^*Corresponding author for this work

National Defense Medical Center Taiwan

Research output: Contribution to journal › Journal Article › peer-review

5 Scopus citations

Abstract

When comparing SV40 T antigen-transformed human fibroblasts of a younger generation (24 population doubling) and aging stage (58 population doubling), we found that detachment of cells from the culture surface occurred more frequently in aging cells. DNA fragmentation and chromatin condensation which are typical findings of apoptosis occurred more frequently in aging cells as compared to cells of a younger generation. There is no increase in the p53 level or decrease in the SV40 T antigen level in aging cells as compared to cells of a younger generation. Retinoic acid treatment which can effectively suppress p21 gene expression did not prevent apoptosis. These findings indicate that apoptosis that occurs due to aging-transformed human fibroblasts is mediated through p53- and p21-independent pathways. Copyright (C) 1998 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	77-82
Number of pages	6
Journal	Cancer Letters
Volume	133
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3835(98)00212-2
State	Published - 13 11 1998
Externally published	Yes

Keywords

Apoptosis
SV40 T antigen
Senescence

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0304-3835(98)00212-2

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title = "Apoptosis is induced in aging SV40 T antigen-transformed human fibroblasts through p53- and p21(CIP1/WAF1)-independent pathways",

abstract = "When comparing SV40 T antigen-transformed human fibroblasts of a younger generation (24 population doubling) and aging stage (58 population doubling), we found that detachment of cells from the culture surface occurred more frequently in aging cells. DNA fragmentation and chromatin condensation which are typical findings of apoptosis occurred more frequently in aging cells as compared to cells of a younger generation. There is no increase in the p53 level or decrease in the SV40 T antigen level in aging cells as compared to cells of a younger generation. Retinoic acid treatment which can effectively suppress p21 gene expression did not prevent apoptosis. These findings indicate that apoptosis that occurs due to aging-transformed human fibroblasts is mediated through p53- and p21-independent pathways. Copyright (C) 1998 Elsevier Science Ireland Ltd.",

keywords = "Apoptosis, SV40 T antigen, Senescence",

author = "Tsao, {Yeou Ping} and Li, {Shu Fang} and Liu, {Jiang Chuan} and Chen, {Show Li}",

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AU - Tsao, Yeou Ping

AU - Li, Shu Fang

AU - Liu, Jiang Chuan

AU - Chen, Show Li

PY - 1998/11/13

Y1 - 1998/11/13

N2 - When comparing SV40 T antigen-transformed human fibroblasts of a younger generation (24 population doubling) and aging stage (58 population doubling), we found that detachment of cells from the culture surface occurred more frequently in aging cells. DNA fragmentation and chromatin condensation which are typical findings of apoptosis occurred more frequently in aging cells as compared to cells of a younger generation. There is no increase in the p53 level or decrease in the SV40 T antigen level in aging cells as compared to cells of a younger generation. Retinoic acid treatment which can effectively suppress p21 gene expression did not prevent apoptosis. These findings indicate that apoptosis that occurs due to aging-transformed human fibroblasts is mediated through p53- and p21-independent pathways. Copyright (C) 1998 Elsevier Science Ireland Ltd.

AB - When comparing SV40 T antigen-transformed human fibroblasts of a younger generation (24 population doubling) and aging stage (58 population doubling), we found that detachment of cells from the culture surface occurred more frequently in aging cells. DNA fragmentation and chromatin condensation which are typical findings of apoptosis occurred more frequently in aging cells as compared to cells of a younger generation. There is no increase in the p53 level or decrease in the SV40 T antigen level in aging cells as compared to cells of a younger generation. Retinoic acid treatment which can effectively suppress p21 gene expression did not prevent apoptosis. These findings indicate that apoptosis that occurs due to aging-transformed human fibroblasts is mediated through p53- and p21-independent pathways. Copyright (C) 1998 Elsevier Science Ireland Ltd.

KW - Apoptosis

KW - SV40 T antigen

KW - Senescence

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DO - 10.1016/S0304-3835(98)00212-2

M3 - 文章

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AN - SCOPUS:0032415293

SN - 0304-3835

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JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Apoptosis is induced in aging SV40 T antigen-transformed human fibroblasts through p53- and p21(CIP1/WAF1)-independent pathways

Abstract

Keywords

UN SDGs

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