Aptamer-based colorimetric detection of platelet-derived growth factor using unmodified gold nanoparticles

Chia Chen Chang, Shih Chung Wei, Tzu Heng Wu, Chung Han Lee, Chii Wann Lin*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

88 Scopus citations

Abstract

We developed a simple method for the detection of platelet-derived growth factors (PDGFs) based on base stacking effect coupled with an unmodified gold nanoparticle (AuNP) indicator. In the absence of a target, an aptamer probe and a capture probe stably co-exist in a solution, as it is difficult to sustain an interaction between both these probes due to the short 8. bp duplex. However, when a target protein binds to the aptamer probe, the strong base stacking effect can lead to a favorable and stable interaction between the aptamer and capture probes. Hence, the capture probe dissociates from the AuNP surfaces, inducing AuNP aggregation. Compared with other AuNP-based aptasensors for PDGFs, using this base stacking effect can overcome a structured-aptamer method's limitation of requiring thiolated-aptamer-modified AuNPs. Under optimal detection conditions, this label-free colorimetric sensor could detect PDGFs down to 6. nM with high selectivity in the presence of other interferring proteins. This simple detection approach provides viable methods for a structured-aptamer sensing protocol.

Original languageEnglish
Pages (from-to)119-123
Number of pages5
JournalBiosensors and Bioelectronics
Volume42
Issue number1
DOIs
StatePublished - 05 04 2013
Externally publishedYes

Keywords

  • Aptamer
  • Base stacking
  • Colorimetry
  • Gold nanoparticle
  • Label-free
  • Platelet-derived growth factor

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