Arsenic-related Bowen's disease and paraquat-related skin cancerous lesions show no detectable ras and p53 gene alterations

Ling Ling Hsieh; Huai Jung Chen; Jen Tsung Hsieh; Shiou Hwa Jee; Gwo Shing Chen; Chien Jen Chen

doi:10.1016/0304-3835(94)90180-5

Arsenic-related Bowen's disease and paraquat-related skin cancerous lesions show no detectable ras and p53 gene alterations

Ling Ling Hsieh^*, Huai Jung Chen, Jen Tsung Hsieh, Shiou Hwa Jee, Gwo Shing Chen, Chien Jen Chen

^*Corresponding author for this work

Department of Public Health

Research output: Contribution to journal › Journal Article › peer-review

31 Scopus citations

Abstract

We have investigated point mutations of condons 12, 13, and 61 in H-, K-, and N-ras oncogenes as well as p53 tumour suppressor gene exon 5 through exon 9 by PCR-SSCP analysis in 26 skin biopsy tissues from 16 arsenic-related Bowen's disease patients and 6 skin samples from 4 paraquat manufacturing workers. No mutation was found. These results are different from findings with UV associated skin cancers. Interestingly, a silent change at condon 27 of H-ras in one allele was detected in all 4 paraquat manufacturing workers and in 2 of 16 arsenic-related Bowen's disease patients. It is likely that the molecular mechanisms involved in arsenic and paraquat induced skin cancers differ from sunlight-related skin maligancies.

Original language	English
Pages (from-to)	59-65
Number of pages	7
Journal	Cancer Letters
Volume	86
Issue number	1
DOIs	https://doi.org/10.1016/0304-3835(94)90180-5
State	Published - 28 10 1994

Keywords

Bowen's disease
PCR-SSCP
p53
ras

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0304-3835(94)90180-5

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title = "Arsenic-related Bowen's disease and paraquat-related skin cancerous lesions show no detectable ras and p53 gene alterations",

abstract = "We have investigated point mutations of condons 12, 13, and 61 in H-, K-, and N-ras oncogenes as well as p53 tumour suppressor gene exon 5 through exon 9 by PCR-SSCP analysis in 26 skin biopsy tissues from 16 arsenic-related Bowen's disease patients and 6 skin samples from 4 paraquat manufacturing workers. No mutation was found. These results are different from findings with UV associated skin cancers. Interestingly, a silent change at condon 27 of H-ras in one allele was detected in all 4 paraquat manufacturing workers and in 2 of 16 arsenic-related Bowen's disease patients. It is likely that the molecular mechanisms involved in arsenic and paraquat induced skin cancers differ from sunlight-related skin maligancies.",

keywords = "Bowen's disease, PCR-SSCP, p53, ras",

author = "Hsieh, {Ling Ling} and Chen, {Huai Jung} and Hsieh, {Jen Tsung} and Jee, {Shiou Hwa} and Chen, {Gwo Shing} and Chen, {Chien Jen}",

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T1 - Arsenic-related Bowen's disease and paraquat-related skin cancerous lesions show no detectable ras and p53 gene alterations

AU - Hsieh, Ling Ling

AU - Chen, Huai Jung

AU - Hsieh, Jen Tsung

AU - Jee, Shiou Hwa

AU - Chen, Gwo Shing

AU - Chen, Chien Jen

PY - 1994/10/28

Y1 - 1994/10/28

N2 - We have investigated point mutations of condons 12, 13, and 61 in H-, K-, and N-ras oncogenes as well as p53 tumour suppressor gene exon 5 through exon 9 by PCR-SSCP analysis in 26 skin biopsy tissues from 16 arsenic-related Bowen's disease patients and 6 skin samples from 4 paraquat manufacturing workers. No mutation was found. These results are different from findings with UV associated skin cancers. Interestingly, a silent change at condon 27 of H-ras in one allele was detected in all 4 paraquat manufacturing workers and in 2 of 16 arsenic-related Bowen's disease patients. It is likely that the molecular mechanisms involved in arsenic and paraquat induced skin cancers differ from sunlight-related skin maligancies.

AB - We have investigated point mutations of condons 12, 13, and 61 in H-, K-, and N-ras oncogenes as well as p53 tumour suppressor gene exon 5 through exon 9 by PCR-SSCP analysis in 26 skin biopsy tissues from 16 arsenic-related Bowen's disease patients and 6 skin samples from 4 paraquat manufacturing workers. No mutation was found. These results are different from findings with UV associated skin cancers. Interestingly, a silent change at condon 27 of H-ras in one allele was detected in all 4 paraquat manufacturing workers and in 2 of 16 arsenic-related Bowen's disease patients. It is likely that the molecular mechanisms involved in arsenic and paraquat induced skin cancers differ from sunlight-related skin maligancies.

KW - Bowen's disease

KW - PCR-SSCP

KW - p53

KW - ras

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Arsenic-related Bowen's disease and paraquat-related skin cancerous lesions show no detectable ras and p53 gene alterations

Abstract

Keywords

UN SDGs

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