Au-nanoarray by nanosphere lithography sensors for staphylococcus aureus 16S rRNA

Agnes Purwidyantr; Chao Sung Lai

doi:10.1109/NANO.2016.7751569

Au-nanoarray by nanosphere lithography sensors for staphylococcus aureus 16S rRNA

Agnes Purwidyantr
, Chao Sung Lai

Department of Electronic Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Nanopatterning of Au-nanoarray on an indium tin oxide (ITO) electrode using efficient and low-cost nanospheres lithography (NSL) is reported. Convective self-assembly of 500 nm polystyrene (PS) nanobeads was found to effectively provide a well-ordered monolayer template for Au deposition with the coverage of about 90% on ITO. A simple Au-nanoparticles (AuNPs) spin coating was performed. It is observed that AuNPs immobilized by spin-coating techniques tends to follow the contour of the spherical nanobeads instead of filling the gaps within them, therefore, a distinguished hexagonal ring of Aunanoarray was generated after nanobeads etching. As used for electrochemical sensor of Staphyloccocus aureus 16S rRNA hybridization detection, this substrate resulted high effective surface area which subsequently gave an excellent limitation of detection (LOD) of 10 pM target DNA. Overall, we have demonstrated an integration of NSL and AuNPs spin-coating for a uniform and high troughput substrate fabrication for electrochemical biosensor development.

Original language	English
Title of host publication	16th International Conference on Nanotechnology - IEEE NANO 2016
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	401-404
Number of pages	4
ISBN (Electronic)	9781509039142
DOIs	https://doi.org/10.1109/NANO.2016.7751569
State	Published - 21 11 2016
Event	16th IEEE International Conference on Nanotechnology - IEEE NANO 2016 - Sendai, Japan Duration: 22 08 2016 → 25 08 2016

Publication series

Name	16th International Conference on Nanotechnology - IEEE NANO 2016

Conference

Conference	16th IEEE International Conference on Nanotechnology - IEEE NANO 2016
Country/Territory	Japan
City	Sendai
Period	22/08/16 → 25/08/16

Bibliographical note

Publisher Copyright:
© 2016 IEEE.

Keywords

16S rRNA
Au-nanoarray
Electrochemical
Nanosphere lithography
S. Aureus
Spin-coating

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10.1109/NANO.2016.7751569

Cite this

Purwidyantr, A., & Lai, C. S. (2016). Au-nanoarray by nanosphere lithography sensors for staphylococcus aureus 16S rRNA. In 16th International Conference on Nanotechnology - IEEE NANO 2016 (pp. 401-404). Article 7751569 (16th International Conference on Nanotechnology - IEEE NANO 2016). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NANO.2016.7751569

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title = "Au-nanoarray by nanosphere lithography sensors for staphylococcus aureus 16S rRNA",

abstract = "Nanopatterning of Au-nanoarray on an indium tin oxide (ITO) electrode using efficient and low-cost nanospheres lithography (NSL) is reported. Convective self-assembly of 500 nm polystyrene (PS) nanobeads was found to effectively provide a well-ordered monolayer template for Au deposition with the coverage of about 90\% on ITO. A simple Au-nanoparticles (AuNPs) spin coating was performed. It is observed that AuNPs immobilized by spin-coating techniques tends to follow the contour of the spherical nanobeads instead of filling the gaps within them, therefore, a distinguished hexagonal ring of Aunanoarray was generated after nanobeads etching. As used for electrochemical sensor of Staphyloccocus aureus 16S rRNA hybridization detection, this substrate resulted high effective surface area which subsequently gave an excellent limitation of detection (LOD) of 10 pM target DNA. Overall, we have demonstrated an integration of NSL and AuNPs spin-coating for a uniform and high troughput substrate fabrication for electrochemical biosensor development.",

keywords = "16S rRNA, Au-nanoarray, Electrochemical, Nanosphere lithography, S. Aureus, Spin-coating",

author = "Agnes Purwidyantr and Lai, \{Chao Sung\}",

note = "Publisher Copyright: {\textcopyright} 2016 IEEE.; 16th IEEE International Conference on Nanotechnology - IEEE NANO 2016 ; Conference date: 22-08-2016 Through 25-08-2016",

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Purwidyantr, A & Lai, CS 2016, Au-nanoarray by nanosphere lithography sensors for staphylococcus aureus 16S rRNA. in 16th International Conference on Nanotechnology - IEEE NANO 2016., 7751569, 16th International Conference on Nanotechnology - IEEE NANO 2016, Institute of Electrical and Electronics Engineers Inc., pp. 401-404, 16th IEEE International Conference on Nanotechnology - IEEE NANO 2016, Sendai, Japan, 22/08/16. https://doi.org/10.1109/NANO.2016.7751569

Au-nanoarray by nanosphere lithography sensors for staphylococcus aureus 16S rRNA. / Purwidyantr, Agnes; Lai, Chao Sung.
16th International Conference on Nanotechnology - IEEE NANO 2016. Institute of Electrical and Electronics Engineers Inc., 2016. p. 401-404 7751569 (16th International Conference on Nanotechnology - IEEE NANO 2016).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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N2 - Nanopatterning of Au-nanoarray on an indium tin oxide (ITO) electrode using efficient and low-cost nanospheres lithography (NSL) is reported. Convective self-assembly of 500 nm polystyrene (PS) nanobeads was found to effectively provide a well-ordered monolayer template for Au deposition with the coverage of about 90% on ITO. A simple Au-nanoparticles (AuNPs) spin coating was performed. It is observed that AuNPs immobilized by spin-coating techniques tends to follow the contour of the spherical nanobeads instead of filling the gaps within them, therefore, a distinguished hexagonal ring of Aunanoarray was generated after nanobeads etching. As used for electrochemical sensor of Staphyloccocus aureus 16S rRNA hybridization detection, this substrate resulted high effective surface area which subsequently gave an excellent limitation of detection (LOD) of 10 pM target DNA. Overall, we have demonstrated an integration of NSL and AuNPs spin-coating for a uniform and high troughput substrate fabrication for electrochemical biosensor development.

AB - Nanopatterning of Au-nanoarray on an indium tin oxide (ITO) electrode using efficient and low-cost nanospheres lithography (NSL) is reported. Convective self-assembly of 500 nm polystyrene (PS) nanobeads was found to effectively provide a well-ordered monolayer template for Au deposition with the coverage of about 90% on ITO. A simple Au-nanoparticles (AuNPs) spin coating was performed. It is observed that AuNPs immobilized by spin-coating techniques tends to follow the contour of the spherical nanobeads instead of filling the gaps within them, therefore, a distinguished hexagonal ring of Aunanoarray was generated after nanobeads etching. As used for electrochemical sensor of Staphyloccocus aureus 16S rRNA hybridization detection, this substrate resulted high effective surface area which subsequently gave an excellent limitation of detection (LOD) of 10 pM target DNA. Overall, we have demonstrated an integration of NSL and AuNPs spin-coating for a uniform and high troughput substrate fabrication for electrochemical biosensor development.

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ER -

Au-nanoarray by nanosphere lithography sensors for staphylococcus aureus 16S rRNA

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