Augmentation of diabetic wound healing and enhancement of collagen content using nanofibrous glucophage-loaded collagen/PLGA scaffold membranes

Cheng Hung Lee, Shang Hung Chang, Wei Jan Chen, Kuo Chun Hung, Yu Huang Lin, Shih Jung Liu*, Ming Jer Hsieh, Jong Hwei S. Pang, Jyuhn Huarng Juang

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

95 Scopus citations

Abstract

This work developed nanofibrous drug-loaded collagen/poly-. d-. l-lactide-glycolide (PLGA) scaffold membranes that provided the sustained release of glucophage for the wounds associated with diabetes. PLGA, glucophage, and collagen were firstly dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol and were spun into nanofibrous membranes by electrospinning. High-performance liquid chromatography assay was used to characterize the in vivo and in vitro release rates of the pharmaceuticals from the membranes. High concentrations of glucophage were released for over three weeks from the nanofibrous membranes. The nanofibrous glucophage-loaded collagen/PLGA membranes were more hydrophilic than collagen/PLGA membranes and exhibited a greater water-containing capacity. The glucophage-loaded collagen/PLGA membranes markedly promoted the healing of diabetic wounds. Moreover, the collagen content of diabetic rats using drug-eluting membranes was higher than that of the control rats, because of the down-regulation of matrix metalloproteinase 9. The experimental results herein suggest that the nanofibrous glucophage-loaded collagen/PLGA membranes had effect for increasing collagen content in treating diabetic wounds and very effective promoters of the healing of such wounds in the early stages.

Original languageEnglish
Pages (from-to)88-97
Number of pages10
JournalJournal of Colloid and Interface Science
Volume439
DOIs
StatePublished - 01 02 2015

Bibliographical note

Publisher Copyright:
© 2014 Elsevier Inc.

Keywords

  • Diabetic wound repair
  • Electrospinning
  • Nanofibrous glucophage-loaded collagen/PLGA membranes
  • Release characteristics

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