Bacteria detection utilizing electrical conductivity

Yi Chun Lu, Ya Shuan Chuang, Yi Yang Chen, An Chi Shu, Hsing Yu Hsu, Hwan You Chang, Tri Rung Yew*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

35 Scopus citations

Abstract

Real-time and specific detection of single bacterium remains a fundamental challenge and draws very much attention. Using test patterns composed of interdigitated Au-electrode arrays modified with antibody, the specific and quantitative detection of the electrical conductivity of a single Escherichia coli (E. coli, JM109) has been carried out in this work. The key is to ensure low background current of the antibody-modified test patterns before bacteria detection (<0.7 pA in this case) and minimize the residual moisture or hydration after E. coli immobilization, such as via the use of 1-min bake at 50 °C prior to electrical measurement. This method holds great potential for future application in the real-time, specific, and quantitative bacterium detection down to a single bacterium cell.

Original languageEnglish
Pages (from-to)1856-1861
Number of pages6
JournalBiosensors and Bioelectronics
Volume23
Issue number12
DOIs
StatePublished - 15 07 2008
Externally publishedYes

Keywords

  • Antibody immobilization
  • Bacteria detection
  • Bacteria immobilization
  • Biosensor
  • Escherichia coli (E. coli
  • Interdigitated electrode array
  • JM109)

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