Abstract
Real-time and specific detection of single bacterium remains a fundamental challenge and draws very much attention. Using test patterns composed of interdigitated Au-electrode arrays modified with antibody, the specific and quantitative detection of the electrical conductivity of a single Escherichia coli (E. coli, JM109) has been carried out in this work. The key is to ensure low background current of the antibody-modified test patterns before bacteria detection (<0.7 pA in this case) and minimize the residual moisture or hydration after E. coli immobilization, such as via the use of 1-min bake at 50 °C prior to electrical measurement. This method holds great potential for future application in the real-time, specific, and quantitative bacterium detection down to a single bacterium cell.
Original language | English |
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Pages (from-to) | 1856-1861 |
Number of pages | 6 |
Journal | Biosensors and Bioelectronics |
Volume | 23 |
Issue number | 12 |
DOIs | |
State | Published - 15 07 2008 |
Externally published | Yes |
Keywords
- Antibody immobilization
- Bacteria detection
- Bacteria immobilization
- Biosensor
- Escherichia coli (E. coli
- Interdigitated electrode array
- JM109)