Bandeiraea (Griffiona) simplicifolia lectin-I, isolectin A4, reacting with Tn (GalNAcα1→Ser/Thr) or galabiose (Galα1→4Gal) containing ligands

Albert M. Wu; June H. Wu; Shuh Chyung Song; Elvin A. Kabat

doi:10.1016/S0014-5793(96)01227-6

Bandeiraea (Griffiona) simplicifolia lectin-I, isolectin A₄, reacting with Tn (GalNAcα1→Ser/Thr) or galabiose (Galα1→4Gal) containing ligands

Albert M. Wu^*, June H. Wu, Shuh Chyung Song, Elvin A. Kabat

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

14 Scopus citations

Abstract

Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A₄(GS I-A₄) reacting with the Tn(GalNAcα1→Ser/Thr) sequence or human blood group P(k) active disaccharide (E, Galα1→4Gal, galabiose) was studied by quantitative precipitin (QPA) and precipitin-inhibition assays. When human blood group P₁ or Tn active glycoproteins were tested by QPA, GS I-A₄ reacted strongly with both the Tn active glycoproteins purified from asialo porcine, ovine and armadillo submandibular glands and a P₁ active glycoprotein isolated from sheep hydatid fluid. They precipitated over 80% of the lectin nitrogen added. The asialo porcine salivary glycoprotein-GS I-A₄ interaction was inhibited by both Tn containing glycopeptides and Galα1→4Gal indicating that GS I-A₄ not only reacts with human blood group A(GalNAcα1→3Gal) and B(Galα1→3Gal) active disaccharides, but also recognizes the Tn sequence and the E(Galα1→4Gal) ligand. From these results, the carbohydrate specificity of GS I-A₄ can be defined as A, Tn ≤ B and E.

Original language	English
Pages (from-to)	183-186
Number of pages	4
Journal	FEBS Letters
Volume	398
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(96)01227-6
State	Published - 02 12 1996
Externally published	Yes

Keywords

Bandeiraea (Griffonia) simplicifolia lectin-I A
Binding property
Sheep hydatid cyst glycoprotein
Tn-active glycoprotein

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10.1016/S0014-5793(96)01227-6

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title = "Bandeiraea (Griffiona) simplicifolia lectin-I, isolectin A4, reacting with Tn (GalNAcα1→Ser/Thr) or galabiose (Galα1→4Gal) containing ligands",

abstract = "Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A4(GS I-A4) reacting with the Tn(GalNAcα1→Ser/Thr) sequence or human blood group P(k) active disaccharide (E, Galα1→4Gal, galabiose) was studied by quantitative precipitin (QPA) and precipitin-inhibition assays. When human blood group P1 or Tn active glycoproteins were tested by QPA, GS I-A4 reacted strongly with both the Tn active glycoproteins purified from asialo porcine, ovine and armadillo submandibular glands and a P1 active glycoprotein isolated from sheep hydatid fluid. They precipitated over 80% of the lectin nitrogen added. The asialo porcine salivary glycoprotein-GS I-A4 interaction was inhibited by both Tn containing glycopeptides and Galα1→4Gal indicating that GS I-A4 not only reacts with human blood group A(GalNAcα1→3Gal) and B(Galα1→3Gal) active disaccharides, but also recognizes the Tn sequence and the E(Galα1→4Gal) ligand. From these results, the carbohydrate specificity of GS I-A4 can be defined as A, Tn ≤ B and E.",

keywords = "Bandeiraea (Griffonia) simplicifolia lectin-I A, Binding property, Sheep hydatid cyst glycoprotein, Tn-active glycoprotein",

author = "Wu, {Albert M.} and Wu, {June H.} and Song, {Shuh Chyung} and Kabat, {Elvin A.}",

year = "1996",

month = dec,

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doi = "10.1016/S0014-5793(96)01227-6",

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AU - Wu, Albert M.

AU - Wu, June H.

AU - Song, Shuh Chyung

AU - Kabat, Elvin A.

PY - 1996/12/2

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N2 - Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A4(GS I-A4) reacting with the Tn(GalNAcα1→Ser/Thr) sequence or human blood group P(k) active disaccharide (E, Galα1→4Gal, galabiose) was studied by quantitative precipitin (QPA) and precipitin-inhibition assays. When human blood group P1 or Tn active glycoproteins were tested by QPA, GS I-A4 reacted strongly with both the Tn active glycoproteins purified from asialo porcine, ovine and armadillo submandibular glands and a P1 active glycoprotein isolated from sheep hydatid fluid. They precipitated over 80% of the lectin nitrogen added. The asialo porcine salivary glycoprotein-GS I-A4 interaction was inhibited by both Tn containing glycopeptides and Galα1→4Gal indicating that GS I-A4 not only reacts with human blood group A(GalNAcα1→3Gal) and B(Galα1→3Gal) active disaccharides, but also recognizes the Tn sequence and the E(Galα1→4Gal) ligand. From these results, the carbohydrate specificity of GS I-A4 can be defined as A, Tn ≤ B and E.

AB - Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A4(GS I-A4) reacting with the Tn(GalNAcα1→Ser/Thr) sequence or human blood group P(k) active disaccharide (E, Galα1→4Gal, galabiose) was studied by quantitative precipitin (QPA) and precipitin-inhibition assays. When human blood group P1 or Tn active glycoproteins were tested by QPA, GS I-A4 reacted strongly with both the Tn active glycoproteins purified from asialo porcine, ovine and armadillo submandibular glands and a P1 active glycoprotein isolated from sheep hydatid fluid. They precipitated over 80% of the lectin nitrogen added. The asialo porcine salivary glycoprotein-GS I-A4 interaction was inhibited by both Tn containing glycopeptides and Galα1→4Gal indicating that GS I-A4 not only reacts with human blood group A(GalNAcα1→3Gal) and B(Galα1→3Gal) active disaccharides, but also recognizes the Tn sequence and the E(Galα1→4Gal) ligand. From these results, the carbohydrate specificity of GS I-A4 can be defined as A, Tn ≤ B and E.

KW - Bandeiraea (Griffonia) simplicifolia lectin-I A

KW - Binding property

KW - Sheep hydatid cyst glycoprotein

KW - Tn-active glycoprotein

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Bandeiraea (Griffiona) simplicifolia lectin-I, isolectin A₄, reacting with Tn (GalNAcα1→Ser/Thr) or galabiose (Galα1→4Gal) containing ligands

Abstract

Keywords

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