BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing

Po Han Chen; Chia I. Lee; Yu Tzu Weng; Woan Yuh Tarn; Yeou Ping Tsao; Ping Chang Kuo; Pang Hung Hsu; Chu Wei Huang; Chiun Sheng Huang; Hsiu Hsiang Lee; June Tai Wu; Show Li Chen

doi:10.1261/rna.034835.112

BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing

Po Han Chen
, Chia I. Lee
, Yu Tzu Weng
, Woan Yuh Tarn
, Yeou Ping Tsao
, Ping Chang Kuo
, Pang Hung Hsu
, Chu Wei Huang
, Chiun Sheng Huang
, Hsiu Hsiang Lee
, June Tai Wu
, Show Li Chen^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

20 Scopus citations

Abstract

Here, we show that dBCAS2 (CG4980, human Breast Carcinoma Amplified Sequence 2 ortholog) is essential for the viability of Drosophila melanogaster. We find that ubiquitous or tissue-specific depletion of dBCAS2 leads to larval lethality, wing deformities, impaired splicing, and apoptosis. More importantly, overexpression of hBCAS2 rescues these defects. Furthermore, the C-terminal coiled-coil domain of hBCAS2 binds directly to CDC5L and recruits hPrp19/PLRG1 to form a core complex for splicing in mammalian cells and can partially restore wing damage induced by knocking down dBCAS2 in flies. In summary, Drosophila and human BCAS2 share a similar function in RNA splicing, which affects cell viability.

Original language	English
Pages (from-to)	208-218
Number of pages	11
Journal	RNA
Volume	19
Issue number	2
DOIs	https://doi.org/10.1261/rna.034835.112
State	Published - 02 2013
Externally published	Yes

Keywords

BCAS2
Drosophila
Prp19 complex
Splicing
Viability

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1261/rna.034835.112

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@article{883f88781021414199121e5812200d8b,

title = "BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing",

abstract = "Here, we show that dBCAS2 (CG4980, human Breast Carcinoma Amplified Sequence 2 ortholog) is essential for the viability of Drosophila melanogaster. We find that ubiquitous or tissue-specific depletion of dBCAS2 leads to larval lethality, wing deformities, impaired splicing, and apoptosis. More importantly, overexpression of hBCAS2 rescues these defects. Furthermore, the C-terminal coiled-coil domain of hBCAS2 binds directly to CDC5L and recruits hPrp19/PLRG1 to form a core complex for splicing in mammalian cells and can partially restore wing damage induced by knocking down dBCAS2 in flies. In summary, Drosophila and human BCAS2 share a similar function in RNA splicing, which affects cell viability.",

keywords = "BCAS2, Drosophila, Prp19 complex, Splicing, Viability",

author = "Chen, \{Po Han\} and Lee, \{Chia I.\} and Weng, \{Yu Tzu\} and Tarn, \{Woan Yuh\} and Tsao, \{Yeou Ping\} and Kuo, \{Ping Chang\} and Hsu, \{Pang Hung\} and Huang, \{Chu Wei\} and Huang, \{Chiun Sheng\} and Lee, \{Hsiu Hsiang\} and Wu, \{June Tai\} and Chen, \{Show Li\}",

year = "2013",

month = feb,

doi = "10.1261/rna.034835.112",

language = "英语",

volume = "19",

pages = "208--218",

journal = "RNA",

issn = "1355-8382",

publisher = "Cold Spring Harbor Laboratory Press",

number = "2",

}

TY - JOUR

T1 - BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing

AU - Chen, Po Han

AU - Lee, Chia I.

AU - Weng, Yu Tzu

AU - Tarn, Woan Yuh

AU - Tsao, Yeou Ping

AU - Kuo, Ping Chang

AU - Hsu, Pang Hung

AU - Huang, Chu Wei

AU - Huang, Chiun Sheng

AU - Lee, Hsiu Hsiang

AU - Wu, June Tai

AU - Chen, Show Li

PY - 2013/2

Y1 - 2013/2

N2 - Here, we show that dBCAS2 (CG4980, human Breast Carcinoma Amplified Sequence 2 ortholog) is essential for the viability of Drosophila melanogaster. We find that ubiquitous or tissue-specific depletion of dBCAS2 leads to larval lethality, wing deformities, impaired splicing, and apoptosis. More importantly, overexpression of hBCAS2 rescues these defects. Furthermore, the C-terminal coiled-coil domain of hBCAS2 binds directly to CDC5L and recruits hPrp19/PLRG1 to form a core complex for splicing in mammalian cells and can partially restore wing damage induced by knocking down dBCAS2 in flies. In summary, Drosophila and human BCAS2 share a similar function in RNA splicing, which affects cell viability.

AB - Here, we show that dBCAS2 (CG4980, human Breast Carcinoma Amplified Sequence 2 ortholog) is essential for the viability of Drosophila melanogaster. We find that ubiquitous or tissue-specific depletion of dBCAS2 leads to larval lethality, wing deformities, impaired splicing, and apoptosis. More importantly, overexpression of hBCAS2 rescues these defects. Furthermore, the C-terminal coiled-coil domain of hBCAS2 binds directly to CDC5L and recruits hPrp19/PLRG1 to form a core complex for splicing in mammalian cells and can partially restore wing damage induced by knocking down dBCAS2 in flies. In summary, Drosophila and human BCAS2 share a similar function in RNA splicing, which affects cell viability.

KW - BCAS2

KW - Drosophila

KW - Prp19 complex

KW - Splicing

KW - Viability

UR - https://www.scopus.com/pages/publications/84872562115

U2 - 10.1261/rna.034835.112

DO - 10.1261/rna.034835.112

M3 - 文章

C2 - 23249746

AN - SCOPUS:84872562115

SN - 1355-8382

VL - 19

SP - 208

EP - 218

JO - RNA

JF - RNA

IS - 2

ER -

BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing

Abstract

Keywords

UN SDGs

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