Berberine reduces leukocyte adhesion to lps-stimulated endothelial cells and vcam-1 expression both in vivo and in vitro

Y. H. Wu, S. Y. Chuang, W. C. Hong, Y. J. Lai, G. J. Chang, J. H.S. Pang*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

34 Scopus citations

Abstract

Leukocyte adhesion to endothelium plays a critical initiating role in inflammation. Berberine, an antiinflammatory natural compound, is known to attenuate lipopolysaccharide (LPS)-induced lung injury and improve survival of endotoxemic animals with mechanism not fully clarified. This study investigated the effects of berberine on the LPS-induced leukocyte-endothelial cell adhesion both in vivo and in vitro. We first established an animal model to observe the in vivo LPS-induced adhesion of leukocytes to the endothelium of venules in the lung tissue dose-dependently. Pretreatment of LPS-stimulated rats with berberine for 1 h reduced the leukocyte-endothelium adhesion and vascular cell adhesion molecule-1 (VCAM-1) expression in lung. Pretreatment of LPS-stimulated vascular endothelial cells with berberine also dose-dependently decreased the number of adhered THP-1 cells and VCAM-1 expression at both RNA and protein levels. Berberine was further confirmed to inhibit the nuclear translocation and DNA binding activity of LPS-activated nuclear factor-kappa B (NF-kappa B). These data demonstrated an additional molecular mechanism for the profound anti-inflammatory effect of berberine.

Original languageEnglish
Pages (from-to)741-750
Number of pages10
JournalInternational Journal of Immunopathology and Pharmacology
Volume25
Issue number3
DOIs
StatePublished - 2012

Keywords

  • Berberine
  • Endothelial cells
  • Lipopolysaccharides
  • Lung injury
  • Nuclear factor-kappa b
  • Vascular cell adhesion molecule-1

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