TY - JOUR
T1 - Bile duct ligation in developing rats
T2 - Temporal progression of liver, kidney, and brain damage
AU - Sheen, Jiunn Ming
AU - Huang, Li Tung
AU - Hsieh, Chih Sung
AU - Chen, Chih Cheng
AU - Wang, Jia Yi
AU - Tain, You Lin
PY - 2010/8
Y1 - 2010/8
N2 - Purpose: Cholestatic liver disease may result in progressive end-stage liver disease and other extrahepatic complications. We explored the temporal progression of bile duct ligation (BDL)-induced cholestasis in developing rats, focusing on brain cognition and liver and kidney pathology, to elucidate whether these findings were associated with asymmetric dimethylarginine and oxidative stress alterations. Materials and Methods: Three groups of young male Sprague-Dawley rats were studied: one group underwent laparotomy (sham), another group underwent laparotomy and BDL for 2 weeks (BDL2), and a third group underwent laparotomy and BDL for 4 weeks (BDL4). Results: The effect of BDL on liver was represented by transforming growth factor β1 levels and histology activity index scores, which were worse in the BDL4 rats than in the BDL2 rats. BDL4 rats also exhibited more severe spatial memory deficits than BDL2 rats. In addition, renal injury was more progressive in BDL4 rats than in BDL2 rats because BDL4 rats displayed higher Cr levels, elevated tubulointerstitial injury scores, neutrophil gelatinase-associated lipocalin, and symmetric dimethylarginine levels. Conclusions: Our findings highlight the fact that young BDL rats exhibit similar trends of progression of liver, kidney, and brain damage. Further studies are needed to better delineate the nature of progression of organ damage in young cholestatic rats.
AB - Purpose: Cholestatic liver disease may result in progressive end-stage liver disease and other extrahepatic complications. We explored the temporal progression of bile duct ligation (BDL)-induced cholestasis in developing rats, focusing on brain cognition and liver and kidney pathology, to elucidate whether these findings were associated with asymmetric dimethylarginine and oxidative stress alterations. Materials and Methods: Three groups of young male Sprague-Dawley rats were studied: one group underwent laparotomy (sham), another group underwent laparotomy and BDL for 2 weeks (BDL2), and a third group underwent laparotomy and BDL for 4 weeks (BDL4). Results: The effect of BDL on liver was represented by transforming growth factor β1 levels and histology activity index scores, which were worse in the BDL4 rats than in the BDL2 rats. BDL4 rats also exhibited more severe spatial memory deficits than BDL2 rats. In addition, renal injury was more progressive in BDL4 rats than in BDL2 rats because BDL4 rats displayed higher Cr levels, elevated tubulointerstitial injury scores, neutrophil gelatinase-associated lipocalin, and symmetric dimethylarginine levels. Conclusions: Our findings highlight the fact that young BDL rats exhibit similar trends of progression of liver, kidney, and brain damage. Further studies are needed to better delineate the nature of progression of organ damage in young cholestatic rats.
KW - Acute kidney injury
KW - Asymmetric dimethylarginine
KW - Bile duct ligation
KW - Cholestasis, cirrhosis
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=77955902072&partnerID=8YFLogxK
U2 - 10.1016/j.jpedsurg.2009.12.019
DO - 10.1016/j.jpedsurg.2009.12.019
M3 - 文章
C2 - 20713215
AN - SCOPUS:77955902072
SN - 0022-3468
VL - 45
SP - 1650
EP - 1658
JO - Journal of Pediatric Surgery
JF - Journal of Pediatric Surgery
IS - 8
ER -