TY - JOUR
T1 - Bioconjugated gold nanodots and nanoparticles for protein assays based on photoluminescence quenching
AU - Huang, Chih Ching
AU - Chiang, Cheng Kang
AU - Lin, Zong Hong
AU - Lee, Kun Hong
AU - Chang, Huan Tsung
PY - 2008/3/1
Y1 - 2008/3/1
N2 - This study describes the first instance of the use of two differently sized Au nanoparticles (Au NPs), acting separately as donor and acceptor, in homogeneous photoluminescence quenching assays developed for the analysis of proteins. Introduction of a breast cancer marker protein, platelet-derived growth factor AA (PDGF AA), to a solution of 11-mercaptoundecanoic acid-protected, 2.0-nm photoluminescent Au nanodots (LAuND) led to the preparation of PDGF AA-LAuND as the donor. Thiol-derivative PDGF binding aptamers (Apt) and 13-nm spherical Au NPs were used to synthesize the Apt-QAuNP acceptor. The photoluminescence of PDGF AA-LAuND at 520 nm decreased when photoluminescence quenching occurred between Apt-QAuNP and PDGF AA-LAuND. We used the PDGF AA-L AuND/Apt-QAuNP-based molecular light switching system to analyze PDGFs and PDGF α-receptor in separate homogeneous solutions. In the presence of PDGFs, the interaction between Apt-QAuNP and PDGF AA-LAuND decreased as a result of competitive reactions between the PDGFs and Apt-QAuNP. Similarly, the interaction between Apt-Q AuNP and PDGF AA-LAuND reduced as a result of competitive reactions between PDGF α-receptor and PDGF AA-LAuND. The limits of detection (LODs) for PDGF AA and PDGF α-receptor were 80 pM and 0.25 nM, respectively, resulting from a low background photoluminescence signal. When using the Apt-QAuNP as selectors for (a) the enrichment of PDGF AA and (b) the removal of matrixes possessing intense background fluorescence from cell media and urine samples, the LOD for PDGF AA decreased to 10 pM. Unlike quantum dots, the LAuND provide the advantages of biocompatibility, ease of bioconjugation, and minimal toxicity.
AB - This study describes the first instance of the use of two differently sized Au nanoparticles (Au NPs), acting separately as donor and acceptor, in homogeneous photoluminescence quenching assays developed for the analysis of proteins. Introduction of a breast cancer marker protein, platelet-derived growth factor AA (PDGF AA), to a solution of 11-mercaptoundecanoic acid-protected, 2.0-nm photoluminescent Au nanodots (LAuND) led to the preparation of PDGF AA-LAuND as the donor. Thiol-derivative PDGF binding aptamers (Apt) and 13-nm spherical Au NPs were used to synthesize the Apt-QAuNP acceptor. The photoluminescence of PDGF AA-LAuND at 520 nm decreased when photoluminescence quenching occurred between Apt-QAuNP and PDGF AA-LAuND. We used the PDGF AA-L AuND/Apt-QAuNP-based molecular light switching system to analyze PDGFs and PDGF α-receptor in separate homogeneous solutions. In the presence of PDGFs, the interaction between Apt-QAuNP and PDGF AA-LAuND decreased as a result of competitive reactions between the PDGFs and Apt-QAuNP. Similarly, the interaction between Apt-Q AuNP and PDGF AA-LAuND reduced as a result of competitive reactions between PDGF α-receptor and PDGF AA-LAuND. The limits of detection (LODs) for PDGF AA and PDGF α-receptor were 80 pM and 0.25 nM, respectively, resulting from a low background photoluminescence signal. When using the Apt-QAuNP as selectors for (a) the enrichment of PDGF AA and (b) the removal of matrixes possessing intense background fluorescence from cell media and urine samples, the LOD for PDGF AA decreased to 10 pM. Unlike quantum dots, the LAuND provide the advantages of biocompatibility, ease of bioconjugation, and minimal toxicity.
UR - http://www.scopus.com/inward/record.url?scp=41149099572&partnerID=8YFLogxK
U2 - 10.1021/ac701998f
DO - 10.1021/ac701998f
M3 - 文章
C2 - 18237154
AN - SCOPUS:41149099572
SN - 0003-2700
VL - 80
SP - 1497
EP - 1504
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 5
ER -
