TY - JOUR
T1 - Bioengineered Braided Micro-Nano (Multiscale) Fibrous Scaffolds for Tendon Reconstruction
AU - Jayasree, Anjana
AU - Kottappally Thankappan, Shalumon
AU - Ramachandran, Retheesh
AU - Sundaram, M. Nivedhitha
AU - Chen, Chih Hao
AU - Mony, Ullas
AU - Chen, Jyh Ping
AU - Jayakumar, Rangasamy
N1 - Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/3/11
Y1 - 2019/3/11
N2 - A braided multiscale fibrous scaffold consisting of aligned PCL micro/collagen-bFGFnano fibers was fabricated (mPCL-nCol-bFGF) to mimic native tendon tissue architecture which was further coated with alginate to aid in prevention of peritendinous adhesion. The bFGF release kinetics showed a sustained release of growth factors for a period of 20 days. Further, in vitro cell viability, attachment, and proliferation were performed using rabbit tenocytes under static and dynamic conditions. mPCL-nCol-bFGF showed a higher cell proliferation and enhanced expression of tenogenic markers compared to mPCL-nCol (braided scaffold without bFGF). When subjected to dynamic stimulation in a bioreactor, mPCL-nCol-bFGF-DS (braided scaffold with bFGF after dynamic stimulation) showed enhanced cellular proliferation and tenogenic marker expression, compared to mPCL-nCol-bFGF. The in vivo studies of the cell seeded scaffold after dynamic stimulation in Achilles tendon defect model showed tendon tissue regeneration with aligned collagen morphology within 12 weeks of implantation.
AB - A braided multiscale fibrous scaffold consisting of aligned PCL micro/collagen-bFGFnano fibers was fabricated (mPCL-nCol-bFGF) to mimic native tendon tissue architecture which was further coated with alginate to aid in prevention of peritendinous adhesion. The bFGF release kinetics showed a sustained release of growth factors for a period of 20 days. Further, in vitro cell viability, attachment, and proliferation were performed using rabbit tenocytes under static and dynamic conditions. mPCL-nCol-bFGF showed a higher cell proliferation and enhanced expression of tenogenic markers compared to mPCL-nCol (braided scaffold without bFGF). When subjected to dynamic stimulation in a bioreactor, mPCL-nCol-bFGF-DS (braided scaffold with bFGF after dynamic stimulation) showed enhanced cellular proliferation and tenogenic marker expression, compared to mPCL-nCol-bFGF. The in vivo studies of the cell seeded scaffold after dynamic stimulation in Achilles tendon defect model showed tendon tissue regeneration with aligned collagen morphology within 12 weeks of implantation.
KW - bFGF
KW - braided scaffold
KW - multiscale fibers
KW - poly(ϵ-caprolactone)
KW - tendon regeneration
UR - http://www.scopus.com/inward/record.url?scp=85062779996&partnerID=8YFLogxK
U2 - 10.1021/acsbiomaterials.8b01328
DO - 10.1021/acsbiomaterials.8b01328
M3 - 文章
AN - SCOPUS:85062779996
SN - 2373-9878
VL - 5
SP - 1476
EP - 1486
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
IS - 3
ER -