Blood Plasma Separation Using a Fidget-Spinner

Chao Hsuan Liu, Chung An Chen, Shi Jia Chen, Tsung Ting Tsai, Chin Chou Chu, Chien Cheng Chang*, Chien Fu Chen

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

57 Scopus citations

Abstract

In this study, we present a simple, hand-powered, and electricity-free centrifuge platform based on a commercially available "fidget-spinner." The centrifugal force provided by this inexpensive and easy-to-use toy is sufficient to separate whole blood, producing a plasma yield rate and purity of 30% and 99%, respectively, separated in as little as 4-7 min. We verified the separated plasma by performing a paper-based HIV-1 p24 capsid protein enzyme-linked immunosorbent assay, which achieved a recovery rate of up to 98%, indicating the plasma features extremely low matrix interference effects. These results demonstrate the reliability of the platform for practical use, in addition to greatly reducing the overall cost and time of analysis while retaining detection precision, making it suitable for medical applications in resource-limited regions of the world.

Original languageEnglish
Pages (from-to)1247-1253
Number of pages7
JournalAnalytical Chemistry
Volume91
Issue number2
DOIs
StatePublished - 15 01 2019

Bibliographical note

Publisher Copyright:
© 2018 American Chemical Society.

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