Borealin/Dasra B is a cell cycle-regulated chromosomal passenger protein and its nuclear accumulation is linked to poor prognosis for human gastric cancer

Junn Liang Chang, Ting Hsuan Chen, Chia Fang Wang, Yi Hsuan Chiang, Ya Ling Huang, Fen Hwa Wong, Chen Kung Chou*, Chun Ming Chen

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

49 Scopus citations

Abstract

Chromosomal passenger proteins including Aurora B, Survivin, and Borealin/Dasra B, also called CDCA8/FLJ10468, are known to play crucial roles during mitosis and cell division. Inappropriate chromosomal segregation and cell division may cause auneuploidy leading to cancer. However, it is still unclear how the expression of chromosomal passenger proteins may be linked to cancer. In this study, we demonstrated that Borealin is a cell cycle-regulated gene and is upregulated at G2-M phases of the cell cycle. We showed that Borealin interacts with Survivin but not with Aurora B. The interaction domain of Survivin in Borealin was mapped to the N-terminal 92 amino-acid residues of Borealin. To examine the linkage between expression of Borealin and cancer, we performed immunohistochemistry analysis using anti-Borealin specific antibody on the paraffin-embedded gastric cancer tissues. Our results showed that Borealin expression is significantly correlated with Survivin (P = 0.003) and Ki67 (P = 0.007) in gastric cancer. Interestingly, an increased nuclear Borealin level reveals borderline association with a poor survival rate (P = 0.047). Taken together, our results demonstrated that Borealin is a cell cycle-regulated chromosomal passenger protein and its aberrant expression is linked to a poor prognosis for gastric cancer.

Original languageEnglish
Pages (from-to)962-973
Number of pages12
JournalExperimental Cell Research
Volume312
Issue number7
DOIs
StatePublished - 15 04 2006

Keywords

  • CDCA8
  • Chromosomal passenger protein
  • Gastric cancer
  • Mitosis
  • Survivin

Fingerprint

Dive into the research topics of 'Borealin/Dasra B is a cell cycle-regulated chromosomal passenger protein and its nuclear accumulation is linked to poor prognosis for human gastric cancer'. Together they form a unique fingerprint.

Cite this