CA10 is associated with HBV-related hepatocarcinogenesis

Kuei Min Chung, Ya Ting Chen, Chih Chen Hong, Il Chi Chang, Si Ying Lin, Li Yu Liang, Yi Rong Chen, Chau Ting Yeh, Shiu Feng Huang*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

1 Scopus citations

Abstract

Hepatocellular carcinoma (HCC) is the main threat for the patients infected with hepatitis B virus (HBV), but the oncogenic mechanism of HBV-related HCC is still controversial. Previously, we have found that several HBV surface gene (HBS) non-sense mutations are oncogenic. Among these mutations, sW182* was found to have the most potent oncogenicity. In this study, we found that Carbonic Anhydrase X (CA10) level was specifically increased in sW182* mutant-expressing cells. CA10 overexpression was also associated with HBS nonsense mutation in HBV-related HCC tumor tissues. Transformation and tumorigenesis assays revealed that CA10 had significant oncogenic activity. In addition, CA10 overexpression resulted in dysregulation of apoptosis-related proteins, including Mcl-1, Bcl-2, Bcl-xL and Bad. While searching for the regulatory mechanism of CA10, miR-27b was found to downregulate CA10 expression by regulating its mRNA degradation and its expression was decreased in sW182* mutant cells. Moreover, CA10 overexpression was associated with down-regulation of miR-27b in human HBV-related HCC tumor tissues with sW182* mutation. Therefore, induction of the expression of CA10 through repression of miR-27b by sW182* might be one mechanism involved in HBS mutation-related hepatocarcinogenesis.

Original languageEnglish
Article number101303
JournalBiochemistry and Biophysics Reports
Volume31
DOIs
StatePublished - 09 2022

Bibliographical note

Publisher Copyright:
© 2022

Keywords

  • CA10
  • HBV
  • HCC
  • W182 nonsense mutation
  • miR-27b

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