TY - JOUR
T1 - Carbohydrate specificity of the receptor sites of mistletoe toxic lectin-I
AU - Wu, Albert M.
AU - Chin, Lee Kian
AU - Franz, Hartmut
AU - Pfüller, Uwe
AU - Herp, Anthony
PY - 1992/9/15
Y1 - 1992/9/15
N2 - The carbohydrate specificity of mistletoe toxic lectin-I (ML-I) was studied by haemagglutination-inhibition assay. The results indicated that ML-I has a broad range of affinity for Gal, α, β linked sequences. The galabiose (C, Galα1→4Gal) sequence, a receptor of the uropathogenic E. coli ligand, was one of the best disaccharide inhibitors tested. The lectin also exhibits affinity for Lac(Galβ1→4Glc), T(Galβ1→3GalNAc), I/II(Galβ1→3/4GlcNAc) and B(Galα1→3Gal) sequences. Galα1→4Gal and Galβ1→4Glc are frequently occurring sequences of many glycosphingolipids located at the mammalian cell membranes, such as intestinal and red blood cell membranes, for ligand binding and toxin attachment. This finding provides important information concerning the possible mechanism of intoxication of cells by the mistletoe preparation.
AB - The carbohydrate specificity of mistletoe toxic lectin-I (ML-I) was studied by haemagglutination-inhibition assay. The results indicated that ML-I has a broad range of affinity for Gal, α, β linked sequences. The galabiose (C, Galα1→4Gal) sequence, a receptor of the uropathogenic E. coli ligand, was one of the best disaccharide inhibitors tested. The lectin also exhibits affinity for Lac(Galβ1→4Glc), T(Galβ1→3GalNAc), I/II(Galβ1→3/4GlcNAc) and B(Galα1→3Gal) sequences. Galα1→4Gal and Galβ1→4Glc are frequently occurring sequences of many glycosphingolipids located at the mammalian cell membranes, such as intestinal and red blood cell membranes, for ligand binding and toxin attachment. This finding provides important information concerning the possible mechanism of intoxication of cells by the mistletoe preparation.
KW - Carbohydrate binding property
KW - Carbohydrate combining site
KW - Haemagglutination-inhibition assay
KW - Mistletoe toxic lectin-I
UR - http://www.scopus.com/inward/record.url?scp=0026665482&partnerID=8YFLogxK
U2 - 10.1016/0304-4165(92)90084-8
DO - 10.1016/0304-4165(92)90084-8
M3 - 文章
C2 - 1525184
AN - SCOPUS:0026665482
SN - 0304-4165
VL - 1117
SP - 232
EP - 234
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
IS - 2
ER -