TY - JOUR
T1 - Carbohydrate structural units in glycosphingolipids as receptors for Gal and GalNac reactive lectins
AU - Wu, Albert M.
PY - 2002/8
Y1 - 2002/8
N2 - Glycosphingolipids (GSLs) contain many carbohydrate epitopes or crypto-glycotopes for Gal and GalNAc reactive lectins. Many of them are in the nervous system and function as important receptors in various life processes. During the past two decades, 11 mammalian structural units have been used to express the binding domain of applied lectins. They are: F, GalNAcα1 → 3GalNAc; A, GalNAcα1 → 3Gal; T, Galβ1 → 3GalNAc; I, Galβ1 → 3GlcNAc; II, Galβ1 → 4GlcNAc; B, Galα1 → 3Gal; E, Galα1 → 4Gal; L, Galβ1 → 4Glc; P, GalNAcβ1 → 3Gal; S, GalNAcβ1 → 4Gal, and Tn, GalNAcα1 → Ser(Thr). Although 10 of them occur in GSLs, only 3 (Lβ, Sβ, and Tβ) are found in human brain, and 2 (Lβ and IIβ) are present in the inner structures of human blood group active GSLs. In the families of gangliosides, Lβ and IIβ represent 55% of the total structural units, while the other three units (Tβ, Pα, and Sβ) constitute the rest. To facilitate the selection of lectins that could serve as structural probes, the carbohydrate binding specificities of Gal/GalNAc reactive lectins have been classified according to their highest affinity for the structural units and their binding properties expressed by decreasing order of reactivity. Hence, the binding relation between GSLs and Gal/GalNAc specific lectins can be established.
AB - Glycosphingolipids (GSLs) contain many carbohydrate epitopes or crypto-glycotopes for Gal and GalNAc reactive lectins. Many of them are in the nervous system and function as important receptors in various life processes. During the past two decades, 11 mammalian structural units have been used to express the binding domain of applied lectins. They are: F, GalNAcα1 → 3GalNAc; A, GalNAcα1 → 3Gal; T, Galβ1 → 3GalNAc; I, Galβ1 → 3GlcNAc; II, Galβ1 → 4GlcNAc; B, Galα1 → 3Gal; E, Galα1 → 4Gal; L, Galβ1 → 4Glc; P, GalNAcβ1 → 3Gal; S, GalNAcβ1 → 4Gal, and Tn, GalNAcα1 → Ser(Thr). Although 10 of them occur in GSLs, only 3 (Lβ, Sβ, and Tβ) are found in human brain, and 2 (Lβ and IIβ) are present in the inner structures of human blood group active GSLs. In the families of gangliosides, Lβ and IIβ represent 55% of the total structural units, while the other three units (Tβ, Pα, and Sβ) constitute the rest. To facilitate the selection of lectins that could serve as structural probes, the carbohydrate binding specificities of Gal/GalNAc reactive lectins have been classified according to their highest affinity for the structural units and their binding properties expressed by decreasing order of reactivity. Hence, the binding relation between GSLs and Gal/GalNAc specific lectins can be established.
KW - Applied lectins
KW - Brain and ganglioside
KW - Disaccharide units
KW - Glycotopes in GSLs
UR - http://www.scopus.com/inward/record.url?scp=0036696936&partnerID=8YFLogxK
U2 - 10.1023/A:1020263730943
DO - 10.1023/A:1020263730943
M3 - 文章
C2 - 12374194
AN - SCOPUS:0036696936
SN - 0364-3190
VL - 27
SP - 593
EP - 600
JO - Neurochemical Research
JF - Neurochemical Research
IS - 7-8
ER -