TY - JOUR
T1 - Carica papaya lipase
T2 - A novel biocatalyst for the enantioselective hydrolysis of (R,S)-naproxen 2,2,2-trifluoroethyl ester
AU - Chen, Chun Chi
AU - Tsai, Shau Wei
PY - 2005/1/6
Y1 - 2005/1/6
N2 - Carica papaya lipase (CPL) stores in the crude papain is explored as a high enantioselective biocatalyst for the hydrolysis resolution of (R,S)-naproxen 2,2,2-trifluoroethyl ester in water-saturated solvents. An optimal temperature of 60°C for obtaining the maximum initial rate for (S)-naproxen ester in isooctane is found, where the lipase possesses high enantioselectivity with E = 122. Less hydropholic solvents of cyclohexane and MTBE than isooctane are vital on decreasing the lipase activity and enantioselectivity. Comparisons of enzyme performances for CPL and lipase MY indicate that CPL is more enantioselective and active for (S)-naproxen ester, and also more stable in water-saturated isooctane at 60°C.
AB - Carica papaya lipase (CPL) stores in the crude papain is explored as a high enantioselective biocatalyst for the hydrolysis resolution of (R,S)-naproxen 2,2,2-trifluoroethyl ester in water-saturated solvents. An optimal temperature of 60°C for obtaining the maximum initial rate for (S)-naproxen ester in isooctane is found, where the lipase possesses high enantioselectivity with E = 122. Less hydropholic solvents of cyclohexane and MTBE than isooctane are vital on decreasing the lipase activity and enantioselectivity. Comparisons of enzyme performances for CPL and lipase MY indicate that CPL is more enantioselective and active for (S)-naproxen ester, and also more stable in water-saturated isooctane at 60°C.
KW - (R,S)-Naproxen 2,2,2-trifluorothioethyl ester
KW - Carica papaya lipase
KW - Hydrolysis
KW - Kinetic resolution
UR - http://www.scopus.com/inward/record.url?scp=9944237836&partnerID=8YFLogxK
U2 - 10.1016/j.enzmictec.2004.07.004
DO - 10.1016/j.enzmictec.2004.07.004
M3 - 文章
AN - SCOPUS:9944237836
SN - 0141-0229
VL - 36
SP - 127
EP - 132
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
IS - 1
ER -