Carica papaya lipase: A novel biocatalyst for the enantioselective hydrolysis of (R,S)-naproxen 2,2,2-trifluoroethyl ester

Chun Chi Chen, Shau Wei Tsai

Research output: Contribution to journalJournal Article peer-review

33 Scopus citations

Abstract

Carica papaya lipase (CPL) stores in the crude papain is explored as a high enantioselective biocatalyst for the hydrolysis resolution of (R,S)-naproxen 2,2,2-trifluoroethyl ester in water-saturated solvents. An optimal temperature of 60°C for obtaining the maximum initial rate for (S)-naproxen ester in isooctane is found, where the lipase possesses high enantioselectivity with E = 122. Less hydropholic solvents of cyclohexane and MTBE than isooctane are vital on decreasing the lipase activity and enantioselectivity. Comparisons of enzyme performances for CPL and lipase MY indicate that CPL is more enantioselective and active for (S)-naproxen ester, and also more stable in water-saturated isooctane at 60°C.

Original languageEnglish
Pages (from-to)127-132
Number of pages6
JournalEnzyme and Microbial Technology
Volume36
Issue number1
DOIs
StatePublished - 06 01 2005
Externally publishedYes

Keywords

  • (R,S)-Naproxen 2,2,2-trifluorothioethyl ester
  • Carica papaya lipase
  • Hydrolysis
  • Kinetic resolution

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