TY - JOUR
T1 - Carotenoid-Biosynthesis Genes as a Genetic Marker for the Purpose of Gene Cloning
AU - Liu, S. T.
PY - 1993/8/31
Y1 - 1993/8/31
N2 - A cloning vector pSL775 (7.0 kb) was constructed using the carotenoid-biosynthesis genes of Erwinia herbicola Eho 13 (ATCC 53489). This vector contained a ColE1 origin, an ampicillin resistant gene, and a total of 11 single cloning sites: Asp718, Ava1, BamHI, BanII, EcoRV, HindIII, KpnI, MluI, NcoI, NotI, and SmaI. Transforming the vector into an Escherichia coli strain could result in pink clones. On the other hand, insertion of a DNA fragment into one of these cloning sites resulted in nonpigmented clones. The color differential between the two types of colonies could be detected visually on agar medium after culturing the cells at 37°C for 18 hours.
AB - A cloning vector pSL775 (7.0 kb) was constructed using the carotenoid-biosynthesis genes of Erwinia herbicola Eho 13 (ATCC 53489). This vector contained a ColE1 origin, an ampicillin resistant gene, and a total of 11 single cloning sites: Asp718, Ava1, BamHI, BanII, EcoRV, HindIII, KpnI, MluI, NcoI, NotI, and SmaI. Transforming the vector into an Escherichia coli strain could result in pink clones. On the other hand, insertion of a DNA fragment into one of these cloning sites resulted in nonpigmented clones. The color differential between the two types of colonies could be detected visually on agar medium after culturing the cells at 37°C for 18 hours.
UR - https://www.scopus.com/pages/publications/0027272969
U2 - 10.1006/bbrc.1993.2038
DO - 10.1006/bbrc.1993.2038
M3 - 文章
C2 - 8395826
AN - SCOPUS:0027272969
SN - 0006-291X
VL - 195
SP - 259
EP - 263
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -