Ca2+ movement and apoptosis induced by deltamethrin in Madin-Darby canine kidney canine renal tubular cells

Fang Jin Liu, Chiang Ting Chou, Jin Shiung Cheng, Hong Tai Chang, Wei Zhe Liang, Chun Chi Kuo, Soong Yu Kuo, Daih Huang Kuo, Pochuen Shieh, Fang Rong Chang, Chung Ren Jan*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

5 Scopus citations

Abstract

This study explored the effect of deltamethrin, a pesticide, on free Ca2+ concentration [Ca2+]i, viability, and apoptosis in Madin-Darby canine kidney (MDCK) canine renal tubular cells. Deltamethrin at concentrations between 10μM and 40μM evoked [Ca2+]i rises in a concentration-dependent manner. The Ca2+ entry was inhibited by nifedipine, econazole, phorbol 12-myristate 13-acetate, and SKF96365. Treatment with the endoplasmic reticulum Ca2+ pump inhibitor 2,5-di-tert-butylhydroquinone (BHQ) in a Ca2+-free medium abolished deltamethrin-induced [Ca2+]i rise. Treatment with deltamethrin also abolished BHQ-induced [Ca2+]i rise. Inhibition of phospholipase C (PLC) activity with U73122 abolished deltamethrin-evoked [Ca2+]i rise. Deltamethrin killed cells at 30-60μM in a concentration-dependent manner. The cytotoxic effect of deltamethrin was not reversed by prechelating cytosolic Ca2+ with the acetoxymethyl ester of 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid. Annexin V/propidium iodide staining data suggest that 30-50μM deltamethrin induced apoptosis. Together, in MDCK renal tubular cells, deltamethrin induced [Ca2+]i rises that involved Ca2+ entry through protein kinase C-mediated store-operated Ca2+ channels, and PLC-dependent Ca2+ release from the endoplasmic reticulum. Deltamethrin also induced Ca2+-independent cell death that might involve apoptosis.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalKaohsiung Journal of Medical Sciences
Volume31
Issue number1
DOIs
StatePublished - 01 01 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2014, Kaohsiung Medical University. Published by Elsevier Taiwan LLC. All rights reserved.

Keywords

  • Apoptosis
  • Ca
  • Deltamethrin
  • Madin-Darby canine kidney
  • Renal cells

Fingerprint

Dive into the research topics of 'Ca2+ movement and apoptosis induced by deltamethrin in Madin-Darby canine kidney canine renal tubular cells'. Together they form a unique fingerprint.

Cite this