Abstract
The efficiency of retinoic acid (RA)-induced differentiation was dependent on the position of HL-60 cells in the cell cycle. Our results demonstrated that cells at the G1/S border were more efficiently induced to differentiate by short exposure to RA than cells at other phases of the cell cycle. Synchronization of cells in G1/S phase by aphidicolin (APH) or mimosine (MIMO) increased the sensitivity of cells to RA short exposure treatment. Pretreatment with sphinganine (SP), a protein kinase C (PKC) inhibitor, potentiated RA-induced cell differentiation. By cell cycle analysis, SP was found to block the cell progression through the G1/S phase. Consequently, cells accumulated in the G1/S phase of the cell cycle. The present data therefore suggest a possible mechanism of action of SP to enhance RA-induced differentiation.
Original language | English |
---|---|
Pages (from-to) | 193-199 |
Number of pages | 7 |
Journal | FEBS Letters |
Volume | 318 |
Issue number | 2 |
DOIs | |
State | Published - 01 03 1993 |
Keywords
- Cell cycle
- Granulocytic differentiation
- Retinoic acid
- Sphinganine