Cell type classification based on specific membrane capacitance and cytoplasm conductivity using microfluidic devices

Y. Zhao, D. Chen, Y. Luo, S. Huang, H. Lee, M. Wu, R. Long, J. Wang*, J. Chen

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This paper presents a microfluidic system enabling cell type classification based on continuous characterization of cellular specific membrane capacitance (Cspecific membrane) and cytoplasm conductivity (σ cytoplasm) In this study, cells were aspirated continuously through a constriction channel with cell elongations and two-frequency impedance profiles measured. Based on a distributed equivalent circuit model, raw data were translated into Cspecific membrane and σcytoplasm. Two lung cancer cell lines of CRL-5803 cells (ncell=489) and CCL-185 cells (ncell=487) were tested, producing C specific membrane of 1.63±0.52 μF/cm2 vs. 2.00±0.60 μF/cm2 (p<0.001) and σ cytoplasm of 0.90±0.19 S/m vs. 0.73±0.17 S/m (p<0.001), suggesting these two lung tumor cell lines can be classified based on electrical properties.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages856-858
Number of pages3
ISBN (Print)9781632666246
StatePublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 27 10 201331 10 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period27/10/1331/10/13

Keywords

  • Cytoplasm conductivity
  • Distributed equivalent circuit model
  • High throughput
  • Microfluidics
  • Single-cell analysis
  • Specific membrane capacitance

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