Changes in corneal basal epithelial phenotypes in an altered basement membrane

I. Jong Wang, Ray Jui Fang Tsai, Lung Kun Yeh, Ryan Yao Nien Tsai, Fung Rong Hu, Winston W.Y. Kao

Research output: Contribution to journalJournal Article peer-review

7 Scopus citations


Background: To examine the corneal epithelial phenotype in an altered basement membrane. Methodology/Principal Findings: Corneas from 9 patients with symptoms of continuous unstable corneal curvature (CUCC) were harvested by penetrating keratoplasty and subjected to histology examination and immunohistochemical staining with transactivating and N-terminally truncated pP63 transcript (DNp63), cytokeratin 3 (Krt3), ATP-binding cassette subfamily G member 2 (ABCG2), connexin 43 (CX43), p38 mitogen-activated protein kinases (p38MAPK), activating protein 2 (TFAP2), and extracellular signal-regulated kinase (Erk1/2) monoclonal antibodies. Positive immunostaining with ABCG2, p38MAPK, and TFAP2 monoclonal antibodies was observed in the basal epithelial cells of CUCC patients, and CX43 and DNp63 were detected in the full-thickness epithelial cells of CUCC patients. Conclusions/Significance: Our results indicate that alteration of the corneal basement membrane induces a dedifferentiation- like phenotype in corneal basal epithelial cells.

Original languageEnglish
Article numbere14537
JournalPLoS ONE
Issue number1
StatePublished - 2011
Externally publishedYes


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