Characteristics of hepatitis B surface antigen produced in yeast

Kong Bung Choo; Sheue Mei Wu; Hsien Hsiung Lee; Szecheng J. Lo

doi:10.1016/0006-291X(85)91784-X

Characteristics of hepatitis B surface antigen produced in yeast

Kong Bung Choo^*
, Sheue Mei Wu
, Hsien Hsiung Lee
, Szecheng J. Lo

^*Corresponding author for this work

Veterans General Hospital-Taipei
National Yang Ming Chiao Tung University

Research output: Contribution to journal › Journal Article › peer-review

6 Scopus citations

Abstract

We have constructed an expression plasmid for regulated expression of the hepatitis B surface antigen gene in yeast using promoter of the yeast Pho5 gene. In the yeast transformants, the monomeric HBsAg (22K dalton) was estimated to constitute approximately 3% of the total proteins. On extraction, the HBsAg was found to have a bouyant density of 1.18 g/ml and an S_W.20 value of 54. Electron microscopy revealed particles of heterogeneous size ranging from 18-28 nm. When the yeast HBsAg was used to immunize guinea pigs, the anti-HBsAg antibodies produced could react with human serum HBsAg.

Original language	English
Pages (from-to)	160-166
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	131
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(85)91784-X
State	Published - 30 08 1985
Externally published	Yes

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SDG 3 Good Health and Well-being

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10.1016/0006-291X(85)91784-X

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title = "Characteristics of hepatitis B surface antigen produced in yeast",

abstract = "We have constructed an expression plasmid for regulated expression of the hepatitis B surface antigen gene in yeast using promoter of the yeast Pho5 gene. In the yeast transformants, the monomeric HBsAg (22K dalton) was estimated to constitute approximately 3\% of the total proteins. On extraction, the HBsAg was found to have a bouyant density of 1.18 g/ml and an SW.20 value of 54. Electron microscopy revealed particles of heterogeneous size ranging from 18-28 nm. When the yeast HBsAg was used to immunize guinea pigs, the anti-HBsAg antibodies produced could react with human serum HBsAg.",

author = "Choo, \{Kong Bung\} and Wu, \{Sheue Mei\} and Lee, \{Hsien Hsiung\} and Lo, \{Szecheng J.\}",

year = "1985",

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doi = "10.1016/0006-291X(85)91784-X",

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T1 - Characteristics of hepatitis B surface antigen produced in yeast

AU - Choo, Kong Bung

AU - Wu, Sheue Mei

AU - Lee, Hsien Hsiung

AU - Lo, Szecheng J.

PY - 1985/8/30

Y1 - 1985/8/30

N2 - We have constructed an expression plasmid for regulated expression of the hepatitis B surface antigen gene in yeast using promoter of the yeast Pho5 gene. In the yeast transformants, the monomeric HBsAg (22K dalton) was estimated to constitute approximately 3% of the total proteins. On extraction, the HBsAg was found to have a bouyant density of 1.18 g/ml and an SW.20 value of 54. Electron microscopy revealed particles of heterogeneous size ranging from 18-28 nm. When the yeast HBsAg was used to immunize guinea pigs, the anti-HBsAg antibodies produced could react with human serum HBsAg.

AB - We have constructed an expression plasmid for regulated expression of the hepatitis B surface antigen gene in yeast using promoter of the yeast Pho5 gene. In the yeast transformants, the monomeric HBsAg (22K dalton) was estimated to constitute approximately 3% of the total proteins. On extraction, the HBsAg was found to have a bouyant density of 1.18 g/ml and an SW.20 value of 54. Electron microscopy revealed particles of heterogeneous size ranging from 18-28 nm. When the yeast HBsAg was used to immunize guinea pigs, the anti-HBsAg antibodies produced could react with human serum HBsAg.

UR - https://www.scopus.com/pages/publications/0022226397

U2 - 10.1016/0006-291X(85)91784-X

DO - 10.1016/0006-291X(85)91784-X

M3 - 文章

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AN - SCOPUS:0022226397

SN - 0006-291X

VL - 131

SP - 160

EP - 166

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Characteristics of hepatitis B surface antigen produced in yeast

Abstract

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