Characteristics of loofa (Luffa cylindrica) sponge as a carrier for plant cell immobilization

Yu Kuo Liu, Minoru Seki*, Hideo Tanaka, Shintaro Furusaki

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

41 Scopus citations

Abstract

The feasibility of using loofa (Luffa cylindrica) sponge as a carrier for the immobilization of plant cells was investigated. The immobilization capacities of suspension-cultured cells of the plant species Coffea arabica, Carthamus tinctorius and Angelica sinensis, were examined to verify the effect of aggregate size and the parts of the loofa sponge used for immobilization. C. arabica cells with large aggregate size were effectively immobilized on the loofa sponge matrix by entrapment within the random lattice network configuration. The capacity of C. arabica cells to immobilize on loofa sponge was high when the aggregate size was above 500 μm. Sponge segments including both the core and peripheral parts (core/peripheral part) were more efficient immobilizers than those consisting only of the core or the peripheral parts. The immobilization capacities, in a 3 d flask incubation of C. arabica cells, were 0.38, 0.32 and 0.26 g immobilized cells/g sponge for core/peripheral, core and peripheral parts of the loofa sponge, respectively. In a bubble column, a high immobilization capacity of 0.42 g immobilized cells/g sponge for C. arabica cells, after 3 d of incubation, was obtained using the core/peripheral part of the sponge. The immobilization capacity of loofa sponge was found to be comparable to that of polyurethane foam regardless of the cultivation time. It is expected that loofa sponge may be used as an excellent carrier for the immobilization of plant cells.

Original languageEnglish
Pages (from-to)416-421
Number of pages6
JournalJournal of Fermentation and Bioengineering
Volume85
Issue number4
DOIs
StatePublished - 1998
Externally publishedYes

Keywords

  • Immobilization
  • Loofa sponge
  • Luffa cylindrica
  • Plant cells

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