Characterization of a UV-damage recognition factor in vitro that is associated with UV resistance in HeLa cells.

Chuck C.-K. Chao

Research output: Contribution to journalJournal Article peer-review

Abstract

We have previously reported a cisplatin-selected HeLa cell line showing cross-resistance to ultraviolet (UV) radiation and overexpression of UV-damage recognition factors (Chao et al., Mol. Cell. Biol., 11, 2075-2080, 1991). Here, we further characterize a UV-damage recognition factor in vitro using a gel mobility shift assay. The results indicate that the damage-recognition factor is (i) localized mostly in the nucleus, (ii) protease-sensitive, (iii) RNA-independent, (iv) active in a wide range of ionic strengths (50-400 mM NaCl), (v) with a high affinity for UV-damaged DNA (50-fold molar excess competitor causes 50% recognition loss), and (vi) resistant to agents and that modify protein conformation (urea and NP-40), but slightly sensitive to CaCl2. The significance of the identified UV-damage recognition factor in the sensitivity or resistance of cells to UV is also discussed.
Original languageAmerican English
Pages (from-to)105-113
JournalMutation research
Volume281
Issue number2
DOIs
StatePublished - 1992

Keywords

  • Chloramphenicol O-Acetyltransferase/metabolism
  • Cisplatin/pharmacology
  • DNA Damage/genetics
  • DNA Repair/genetics
  • DNA-Binding Proteins/drug effects
  • DNA-Binding Proteins/metabolism
  • DNA/radiation effects
  • Electric Stimulation
  • HeLa Cells/radiation effects
  • Humans
  • Nuclear Proteins/drug effects
  • Nuclear Proteins/metabolism
  • Plasmids/genetics
  • Protein Conformation/drug effects
  • Ultraviolet Rays

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