Characterization of and hormonal effects on subcellular fractions from xanthophores of the goldfish Carassiusauratus l

  • Thomas J. Lynch*
  • , Szecheng J. Lo
  • , John D. Taylor
  • , T. T. Tchen
  • *Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

11 Scopus citations

Abstract

A method is described for the subcellular fractionation of goldfish xanthophores. The procedure produces relatively pure fractions of caroteniod droplets, pterinosomes, cytosol and what appears to be plasma membrane. The presence of a distinct pattern of proteins is shown to be associated with the carotenoid droplets. Treatment of the xanthophores with ACTH affects the buoyant density of some carotenoid droplets and stimulates the phosphorylation of a polypeptide associated with the carotenoid droplets.

Original languageEnglish
Pages (from-to)127-134
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume102
Issue number1
DOIs
StatePublished - 16 09 1981
Externally publishedYes

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