Abstract
In this study, we examine the preparation of chitosan-coated magnetic nanoparticle (MNP) (chitosan-MNP) and the feasibility of using tissue plasminogen activator (tPA) covalently bound to chitosan-MNP surface (chitosan-MNP-tPA) for magnetic targeted delivery of the thrombolytic drug. The physicochemical properties of chitosan-MNP prepared with increasing chitosan/MNP ratios were characterized in detail. The optimum drug loading is reached when 0.5 mg tPA is conjugated with 5 mg chitosan-MNP where 95% drug is attached to the carrier with full retention of its thrombolytic activity. Under magnetic guidance, chitosan-MNP-tPA can reduce the blood clot lysis time by 58% compared with runs without magnetic targeting or by 53% compared with free tPA using the same drug dosage. Effective thrombolysis in response to chitosan-MNP-tPA under magnetic guidance is also demonstrated in a rat embolic model where one-fifth of the dose of tPA may exert similar thrombolytic efficacy of the drug.
| Original language | English |
|---|---|
| Pages (from-to) | 364-372 |
| Number of pages | 9 |
| Journal | Carbohydrate Polymers |
| Volume | 84 |
| Issue number | 1 |
| DOIs | |
| State | Published - 11 02 2011 |
Keywords
- Chitosan
- Drug delivery
- Magnetic nanoparticles
- Thrombolysis
- Tissue plasminogen activator