Characterization of interleukin 8 in woodchucks with chronic hepatitis B and hepatocellular carcinoma

C. J. Liu; Y. J. Huang; H. L. Chen; H. T. Lin; H. L. Lin; M. L. Kuo; J. L. Huang; D. S. Chen; P. J. Chen; H. L. Wu

doi:10.1038/gene.2008.86

Characterization of interleukin 8 in woodchucks with chronic hepatitis B and hepatocellular carcinoma

C. J. Liu, Y. J. Huang, H. L. Chen, H. T. Lin, H. L. Lin, M. L. Kuo, J. L. Huang, D. S. Chen, P. J. Chen, H. L. Wu^*

^*Corresponding author for this work

Department of Microbiology and Immunology

Research output: Contribution to journal › Journal Article › peer-review

4 Scopus citations

Abstract

The eastern woodchuck, Marmota monax, represents a useful animal model to study hepatitis B virus infection in humans. However, immunological studies in this model have been impeded by a lack of basic information about the components of the immune system such as cytokines and chemokines. To clarify the role(s) of interleukin 8 (IL-8) in chronic hepatitis B and hepatocellular carcinoma (HCC) in the woodchuck model, we cloned and characterized the woodchuck IL-8 cDNA and genomic DNA. Sequence analysis revealed that the organization of the wk-IL-8 gene is similar to that of the human IL-8 gene and consists of four exons and three introns. Woodchuck IL-8 protein exhibits the conserved ELRCXC motif of IL-8 and shows 87, 82, 82 and 79% similarity with rabbit, ovine, bovine and human IL-8 proteins, respectively. The biological activity of wk-IL-8 was demonstrated using neutrophil chemotaxis assays. Wk-IL-8 could be readily detected in both tumor and non-tumor tissues with higher expression in the non-tumor tissues in most cases. The results from this study will facilitate the investigation of IL-8 in the immunopathogenesis of hepadnavirus-related diseases by the woodchuck model.

Original language	English
Pages (from-to)	27-36
Number of pages	10
Journal	Genes and Immunity
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/gene.2008.86
State	Published - 2009

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@article{cf296ccf11444f26a41d070fded848d0,

title = "Characterization of interleukin 8 in woodchucks with chronic hepatitis B and hepatocellular carcinoma",

abstract = "The eastern woodchuck, Marmota monax, represents a useful animal model to study hepatitis B virus infection in humans. However, immunological studies in this model have been impeded by a lack of basic information about the components of the immune system such as cytokines and chemokines. To clarify the role(s) of interleukin 8 (IL-8) in chronic hepatitis B and hepatocellular carcinoma (HCC) in the woodchuck model, we cloned and characterized the woodchuck IL-8 cDNA and genomic DNA. Sequence analysis revealed that the organization of the wk-IL-8 gene is similar to that of the human IL-8 gene and consists of four exons and three introns. Woodchuck IL-8 protein exhibits the conserved ELRCXC motif of IL-8 and shows 87, 82, 82 and 79% similarity with rabbit, ovine, bovine and human IL-8 proteins, respectively. The biological activity of wk-IL-8 was demonstrated using neutrophil chemotaxis assays. Wk-IL-8 could be readily detected in both tumor and non-tumor tissues with higher expression in the non-tumor tissues in most cases. The results from this study will facilitate the investigation of IL-8 in the immunopathogenesis of hepadnavirus-related diseases by the woodchuck model.",

author = "Liu, {C. J.} and Huang, {Y. J.} and Chen, {H. L.} and Lin, {H. T.} and Lin, {H. L.} and Kuo, {M. L.} and Huang, {J. L.} and Chen, {D. S.} and Chen, {P. J.} and Wu, {H. L.}",

year = "2009",

doi = "10.1038/gene.2008.86",

language = "英语",

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TY - JOUR

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AU - Liu, C. J.

AU - Huang, Y. J.

AU - Chen, H. L.

AU - Lin, H. T.

AU - Lin, H. L.

AU - Kuo, M. L.

AU - Huang, J. L.

AU - Chen, D. S.

AU - Chen, P. J.

AU - Wu, H. L.

PY - 2009

Y1 - 2009

N2 - The eastern woodchuck, Marmota monax, represents a useful animal model to study hepatitis B virus infection in humans. However, immunological studies in this model have been impeded by a lack of basic information about the components of the immune system such as cytokines and chemokines. To clarify the role(s) of interleukin 8 (IL-8) in chronic hepatitis B and hepatocellular carcinoma (HCC) in the woodchuck model, we cloned and characterized the woodchuck IL-8 cDNA and genomic DNA. Sequence analysis revealed that the organization of the wk-IL-8 gene is similar to that of the human IL-8 gene and consists of four exons and three introns. Woodchuck IL-8 protein exhibits the conserved ELRCXC motif of IL-8 and shows 87, 82, 82 and 79% similarity with rabbit, ovine, bovine and human IL-8 proteins, respectively. The biological activity of wk-IL-8 was demonstrated using neutrophil chemotaxis assays. Wk-IL-8 could be readily detected in both tumor and non-tumor tissues with higher expression in the non-tumor tissues in most cases. The results from this study will facilitate the investigation of IL-8 in the immunopathogenesis of hepadnavirus-related diseases by the woodchuck model.

AB - The eastern woodchuck, Marmota monax, represents a useful animal model to study hepatitis B virus infection in humans. However, immunological studies in this model have been impeded by a lack of basic information about the components of the immune system such as cytokines and chemokines. To clarify the role(s) of interleukin 8 (IL-8) in chronic hepatitis B and hepatocellular carcinoma (HCC) in the woodchuck model, we cloned and characterized the woodchuck IL-8 cDNA and genomic DNA. Sequence analysis revealed that the organization of the wk-IL-8 gene is similar to that of the human IL-8 gene and consists of four exons and three introns. Woodchuck IL-8 protein exhibits the conserved ELRCXC motif of IL-8 and shows 87, 82, 82 and 79% similarity with rabbit, ovine, bovine and human IL-8 proteins, respectively. The biological activity of wk-IL-8 was demonstrated using neutrophil chemotaxis assays. Wk-IL-8 could be readily detected in both tumor and non-tumor tissues with higher expression in the non-tumor tissues in most cases. The results from this study will facilitate the investigation of IL-8 in the immunopathogenesis of hepadnavirus-related diseases by the woodchuck model.

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Characterization of interleukin 8 in woodchucks with chronic hepatitis B and hepatocellular carcinoma

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