Characterization of porcine arterial endothelial cells cultured on amniotic membrane, a potential matrix for vascular tissue engineering

Shu Huai Tsai; Yi Wen Liu; Wei Chun Tang; Zhi Wei Zhou; Chih Yung Hwang; Guang Yuh Hwang; Bor Rung Ou; Cheng Po Hu; Vivian C. Yang; Jan Kan Chen

doi:10.1016/j.bbrc.2007.04.047

Characterization of porcine arterial endothelial cells cultured on amniotic membrane, a potential matrix for vascular tissue engineering

Shu Huai Tsai, Yi Wen Liu, Wei Chun Tang, Zhi Wei Zhou, Chih Yung Hwang, Guang Yuh Hwang, Bor Rung Ou, Cheng Po Hu, Vivian C. Yang^*, Jan Kan Chen

^*Corresponding author for this work

Department of Physiology

Tunghai University

Research output: Contribution to journal › Journal Article › peer-review

37 Scopus citations

Abstract

The existing of basement membrane improves the development of endothelium while constructing blood vessel equivalent. The amniotic membrane (AM) provides a natural basement membrane and has been used in ocular surface reconstruction. This study evaluated the molecular and cellular characteristics of porcine vascular endothelial cells (ECs) cultured on AM. ECs cultured on AM expressed the endothelial marker vWF and exhibited normal endothelial morphology. Here, we demonstrated that AM enhanced the expression of intercellular molecules, platelet-endothelial cell adhesion molecule-1 (PECAM-1), and adhesion molecule VE-cadherin at the intercellular junctions. The expression level of integrin was markedly higher in ECs cultured on AM than on plastic dish. Furthermore, the AM downregulated the expression of E-selectin and P-selectin in both LPS-activated and non-activated ECs. Consistently, adhesion of leukocytes to both activated and non-activated cells was decreased in ECs cultured on AM. Our results suggest that AM is an ideal matrix to develop a functional endothelium in blood vessel equivalent construction.

Original language	English
Pages (from-to)	984-990
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	357
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2007.04.047
State	Published - 15 06 2007

Keywords

Amniotic membrane
Cell adhesion molecules
Endothelial cell
Matrix

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10.1016/j.bbrc.2007.04.047

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Tsai, S. H., Liu, Y. W., Tang, W. C., Zhou, Z. W., Hwang, C. Y., Hwang, G. Y., Ou, B. R., Hu, C. P., Yang, V. C., & Chen, J. K. (2007). Characterization of porcine arterial endothelial cells cultured on amniotic membrane, a potential matrix for vascular tissue engineering. Biochemical and Biophysical Research Communications, 357(4), 984-990. https://doi.org/10.1016/j.bbrc.2007.04.047

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title = "Characterization of porcine arterial endothelial cells cultured on amniotic membrane, a potential matrix for vascular tissue engineering",

abstract = "The existing of basement membrane improves the development of endothelium while constructing blood vessel equivalent. The amniotic membrane (AM) provides a natural basement membrane and has been used in ocular surface reconstruction. This study evaluated the molecular and cellular characteristics of porcine vascular endothelial cells (ECs) cultured on AM. ECs cultured on AM expressed the endothelial marker vWF and exhibited normal endothelial morphology. Here, we demonstrated that AM enhanced the expression of intercellular molecules, platelet-endothelial cell adhesion molecule-1 (PECAM-1), and adhesion molecule VE-cadherin at the intercellular junctions. The expression level of integrin was markedly higher in ECs cultured on AM than on plastic dish. Furthermore, the AM downregulated the expression of E-selectin and P-selectin in both LPS-activated and non-activated ECs. Consistently, adhesion of leukocytes to both activated and non-activated cells was decreased in ECs cultured on AM. Our results suggest that AM is an ideal matrix to develop a functional endothelium in blood vessel equivalent construction.",

keywords = "Amniotic membrane, Cell adhesion molecules, Endothelial cell, Matrix",

author = "Tsai, {Shu Huai} and Liu, {Yi Wen} and Tang, {Wei Chun} and Zhou, {Zhi Wei} and Hwang, {Chih Yung} and Hwang, {Guang Yuh} and Ou, {Bor Rung} and Hu, {Cheng Po} and Yang, {Vivian C.} and Chen, {Jan Kan}",

year = "2007",

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doi = "10.1016/j.bbrc.2007.04.047",

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AU - Tsai, Shu Huai

AU - Liu, Yi Wen

AU - Tang, Wei Chun

AU - Zhou, Zhi Wei

AU - Hwang, Chih Yung

AU - Hwang, Guang Yuh

AU - Ou, Bor Rung

AU - Hu, Cheng Po

AU - Yang, Vivian C.

AU - Chen, Jan Kan

PY - 2007/6/15

Y1 - 2007/6/15

N2 - The existing of basement membrane improves the development of endothelium while constructing blood vessel equivalent. The amniotic membrane (AM) provides a natural basement membrane and has been used in ocular surface reconstruction. This study evaluated the molecular and cellular characteristics of porcine vascular endothelial cells (ECs) cultured on AM. ECs cultured on AM expressed the endothelial marker vWF and exhibited normal endothelial morphology. Here, we demonstrated that AM enhanced the expression of intercellular molecules, platelet-endothelial cell adhesion molecule-1 (PECAM-1), and adhesion molecule VE-cadherin at the intercellular junctions. The expression level of integrin was markedly higher in ECs cultured on AM than on plastic dish. Furthermore, the AM downregulated the expression of E-selectin and P-selectin in both LPS-activated and non-activated ECs. Consistently, adhesion of leukocytes to both activated and non-activated cells was decreased in ECs cultured on AM. Our results suggest that AM is an ideal matrix to develop a functional endothelium in blood vessel equivalent construction.

AB - The existing of basement membrane improves the development of endothelium while constructing blood vessel equivalent. The amniotic membrane (AM) provides a natural basement membrane and has been used in ocular surface reconstruction. This study evaluated the molecular and cellular characteristics of porcine vascular endothelial cells (ECs) cultured on AM. ECs cultured on AM expressed the endothelial marker vWF and exhibited normal endothelial morphology. Here, we demonstrated that AM enhanced the expression of intercellular molecules, platelet-endothelial cell adhesion molecule-1 (PECAM-1), and adhesion molecule VE-cadherin at the intercellular junctions. The expression level of integrin was markedly higher in ECs cultured on AM than on plastic dish. Furthermore, the AM downregulated the expression of E-selectin and P-selectin in both LPS-activated and non-activated ECs. Consistently, adhesion of leukocytes to both activated and non-activated cells was decreased in ECs cultured on AM. Our results suggest that AM is an ideal matrix to develop a functional endothelium in blood vessel equivalent construction.

KW - Amniotic membrane

KW - Cell adhesion molecules

KW - Endothelial cell

KW - Matrix

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AN - SCOPUS:34247857356

SN - 0006-291X

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Characterization of porcine arterial endothelial cells cultured on amniotic membrane, a potential matrix for vascular tissue engineering

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