Characterization of the response of dendritic cells and regulatory T cells to tumor antigens in patients with renal cell carcinoma

Ming Mo Hou, John Wen Cheng Chang, See Tong Pang, Yang Jen Chiang, Yung Chi Shen, Shuen Kuei Liao, Jia Juan Hsieh, Kun Yun Yeh, Nai Jen Chang, Cheng Keng Chuang*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

6 Scopus citations

Abstract

Background: This study characterized dendritic cells (DCs), regulatory T cells (Tregs) and the immune responses to tumor antigens in renal cell carcinoma (RCC) patients. Methods: Thirty patients with RCC and five healthy donors were studied. DCs were generated from the adherent cells among peripheral blood mononuclear cells (PBMCs), then cultured in medium containing granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 for 7 days. The phenotypes of the DCs and Tregs were analyzed by flow cytometry. A mixed lymphocyte reaction (MLR) was performed to assess the functioning of the DCs and Tregs. A cytotoxic assay was performed to measure the antigen presentation ability of the DCs from the RCC patients (RCC-DCs). These DCs were pretreated with TNF-á (TNF-DCs) or tumor lysate (TuLy-DCs) on the 3rd day of DC culture. Results: The RCC-DCs expressed significantly less CD40 (p = 0.03) and CD80 (p = 0.007) upon TNF-á cultivation than the DCs from healthy donors. The peripheral Tregs during stage I disease were significantly less (p = 0.032) than during stages II-IV. The RCC-DCs were as efficient as DCs from healthy donors (p = 0.83) when stimulating the proliferation of allogeneic T cells; however, these RCC-DCs were less efficient when stimulating autologous T cells than allogeneic T cells (p = 0.023). Tregs inhibited autologous T cell proliferation rather than allogeneic T cell proliferation in response to TuLy-DCs stimulation. Prostaglandin E2 did not increase the ability of immature DCs to stimulate T cell proliferation. Conclusions: Patients with RCC have less potent anti-tumor immune responses.

Original languageEnglish
Pages (from-to)25-35
Number of pages11
JournalChang Gung Medical Journal
Volume33
Issue number1
StatePublished - 01 2010

Keywords

  • Dendritic cells
  • Peripheral blood
  • Regulatory T cells
  • Renal cell carcinoma

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