Chronic intermittent hypoxia stimulates testosterone production in rat Leydig cells

Yu Min Cho; Jou Chun Chou; Chia Mei Fang; S. Hu; Kai Lee Wang; Shyi Wu Wang; Paulus S. Wang

doi:10.1016/j.lfs.2019.116694

Chronic intermittent hypoxia stimulates testosterone production in rat Leydig cells

Yu Min Cho, Jou Chun Chou, Chia Mei Fang, S. Hu, Kai Lee Wang^*, Shyi Wu Wang, Paulus S. Wang

^*Corresponding author for this work

Department of Physiology

Research output: Contribution to journal › Journal Article › peer-review

12 Scopus citations

Abstract

Aims: The hypoxia-stimulated response of the endocrine system depends on the kind and duration of hypoxia. Hypoxia has been reported to stimulate testosterone (T) production in rats, but the mechanisms remain to be investigated. Materials and methods: Male rats were divided into two groups. The rats exposed to chronic intermittent hypoxia (CIH) at 8 h/day were housed in a hypoxic chamber (12% O₂) for 14 days. Normoxic rats were used as control animals. T was measured after challenging the rat Leydig cells (LCs) with different stimulators, including hCG (0.01 IU/ml), forskolin (10⁻ ⁵ M), 8-bromo-cAMP (10⁻ ⁴ M), A23187 (10⁻ ⁵ M), cyclopiazonic acid (10⁻ ⁴ M), and androstenedione (10⁻ ⁸ M). Meanwhile, the LCs were incubated with trilostane (10⁻ ⁵ M) and/or 25-OH-hydroxycholesterol (10⁻ ⁵ M); thereafter the media were collected for pregnenolone assay. Key findings: In the CIH group, plasma T levels were increased, but the serum luteinizing hormone (LH) was decreased. Furthermore, at several time intervals after hCG injection, plasma T levels were higher in the CIH group. The evoked-release of T and pregnenolone were significantly increased in the CIH group. Compared with the normoxic group, the CIH group had higher mRNA and protein expression levels of the LH receptor and CYP11A1 but not StAR. The plasma and testicular microvasculature VEGF levels were increased in the CIH group. The testicular vessel distribution was more obvious in CIH rats. Significance: CIH-induced T secretion might be partially mediated by mechanisms involving the induction of LH receptor expression, testicular angiogenesis, CYP11A1 activity, 17β-HSD activity, and calcium-related pathway.

Original language	English
Article number	116694
Journal	Life Sciences
Volume	233
DOIs	https://doi.org/10.1016/j.lfs.2019.116694
State	Published - 15 09 2019

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Inc.

Keywords

CYP11A1 (P450scc)
Chronic intermittent hypoxia (CIH)
Luteinizing hormone receptor
Testosterone (T)

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10.1016/j.lfs.2019.116694

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@article{198d58890c694f4d95c05e240afeb459,

title = "Chronic intermittent hypoxia stimulates testosterone production in rat Leydig cells",

abstract = "Aims: The hypoxia-stimulated response of the endocrine system depends on the kind and duration of hypoxia. Hypoxia has been reported to stimulate testosterone (T) production in rats, but the mechanisms remain to be investigated. Materials and methods: Male rats were divided into two groups. The rats exposed to chronic intermittent hypoxia (CIH) at 8 h/day were housed in a hypoxic chamber (12\% O2) for 14 days. Normoxic rats were used as control animals. T was measured after challenging the rat Leydig cells (LCs) with different stimulators, including hCG (0.01 IU/ml), forskolin (10− 5 M), 8-bromo-cAMP (10− 4 M), A23187 (10− 5 M), cyclopiazonic acid (10− 4 M), and androstenedione (10− 8 M). Meanwhile, the LCs were incubated with trilostane (10− 5 M) and/or 25-OH-hydroxycholesterol (10− 5 M); thereafter the media were collected for pregnenolone assay. Key findings: In the CIH group, plasma T levels were increased, but the serum luteinizing hormone (LH) was decreased. Furthermore, at several time intervals after hCG injection, plasma T levels were higher in the CIH group. The evoked-release of T and pregnenolone were significantly increased in the CIH group. Compared with the normoxic group, the CIH group had higher mRNA and protein expression levels of the LH receptor and CYP11A1 but not StAR. The plasma and testicular microvasculature VEGF levels were increased in the CIH group. The testicular vessel distribution was more obvious in CIH rats. Significance: CIH-induced T secretion might be partially mediated by mechanisms involving the induction of LH receptor expression, testicular angiogenesis, CYP11A1 activity, 17β-HSD activity, and calcium-related pathway.",

keywords = "CYP11A1 (P450scc), Chronic intermittent hypoxia (CIH), Luteinizing hormone receptor, Testosterone (T)",

author = "Cho, \{Yu Min\} and Chou, \{Jou Chun\} and Fang, \{Chia Mei\} and S. Hu and Wang, \{Kai Lee\} and Wang, \{Shyi Wu\} and Wang, \{Paulus S.\}",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",

year = "2019",

month = sep,

day = "15",

doi = "10.1016/j.lfs.2019.116694",

language = "英语",

volume = "233",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Chronic intermittent hypoxia stimulates testosterone production in rat Leydig cells

AU - Cho, Yu Min

AU - Chou, Jou Chun

AU - Fang, Chia Mei

AU - Hu, S.

AU - Wang, Kai Lee

AU - Wang, Shyi Wu

AU - Wang, Paulus S.

PY - 2019/9/15

Y1 - 2019/9/15

N2 - Aims: The hypoxia-stimulated response of the endocrine system depends on the kind and duration of hypoxia. Hypoxia has been reported to stimulate testosterone (T) production in rats, but the mechanisms remain to be investigated. Materials and methods: Male rats were divided into two groups. The rats exposed to chronic intermittent hypoxia (CIH) at 8 h/day were housed in a hypoxic chamber (12% O2) for 14 days. Normoxic rats were used as control animals. T was measured after challenging the rat Leydig cells (LCs) with different stimulators, including hCG (0.01 IU/ml), forskolin (10− 5 M), 8-bromo-cAMP (10− 4 M), A23187 (10− 5 M), cyclopiazonic acid (10− 4 M), and androstenedione (10− 8 M). Meanwhile, the LCs were incubated with trilostane (10− 5 M) and/or 25-OH-hydroxycholesterol (10− 5 M); thereafter the media were collected for pregnenolone assay. Key findings: In the CIH group, plasma T levels were increased, but the serum luteinizing hormone (LH) was decreased. Furthermore, at several time intervals after hCG injection, plasma T levels were higher in the CIH group. The evoked-release of T and pregnenolone were significantly increased in the CIH group. Compared with the normoxic group, the CIH group had higher mRNA and protein expression levels of the LH receptor and CYP11A1 but not StAR. The plasma and testicular microvasculature VEGF levels were increased in the CIH group. The testicular vessel distribution was more obvious in CIH rats. Significance: CIH-induced T secretion might be partially mediated by mechanisms involving the induction of LH receptor expression, testicular angiogenesis, CYP11A1 activity, 17β-HSD activity, and calcium-related pathway.

AB - Aims: The hypoxia-stimulated response of the endocrine system depends on the kind and duration of hypoxia. Hypoxia has been reported to stimulate testosterone (T) production in rats, but the mechanisms remain to be investigated. Materials and methods: Male rats were divided into two groups. The rats exposed to chronic intermittent hypoxia (CIH) at 8 h/day were housed in a hypoxic chamber (12% O2) for 14 days. Normoxic rats were used as control animals. T was measured after challenging the rat Leydig cells (LCs) with different stimulators, including hCG (0.01 IU/ml), forskolin (10− 5 M), 8-bromo-cAMP (10− 4 M), A23187 (10− 5 M), cyclopiazonic acid (10− 4 M), and androstenedione (10− 8 M). Meanwhile, the LCs were incubated with trilostane (10− 5 M) and/or 25-OH-hydroxycholesterol (10− 5 M); thereafter the media were collected for pregnenolone assay. Key findings: In the CIH group, plasma T levels were increased, but the serum luteinizing hormone (LH) was decreased. Furthermore, at several time intervals after hCG injection, plasma T levels were higher in the CIH group. The evoked-release of T and pregnenolone were significantly increased in the CIH group. Compared with the normoxic group, the CIH group had higher mRNA and protein expression levels of the LH receptor and CYP11A1 but not StAR. The plasma and testicular microvasculature VEGF levels were increased in the CIH group. The testicular vessel distribution was more obvious in CIH rats. Significance: CIH-induced T secretion might be partially mediated by mechanisms involving the induction of LH receptor expression, testicular angiogenesis, CYP11A1 activity, 17β-HSD activity, and calcium-related pathway.

KW - CYP11A1 (P450scc)

KW - Chronic intermittent hypoxia (CIH)

KW - Luteinizing hormone receptor

KW - Testosterone (T)

UR - https://www.scopus.com/pages/publications/85070186193

U2 - 10.1016/j.lfs.2019.116694

DO - 10.1016/j.lfs.2019.116694

M3 - 文章

C2 - 31351970

AN - SCOPUS:85070186193

SN - 0024-3205

VL - 233

JO - Life Sciences

JF - Life Sciences

M1 - 116694

ER -

Chronic intermittent hypoxia stimulates testosterone production in rat Leydig cells

Abstract

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Keywords

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