Circulating microparticles enhanced rat vascular wall remodeling following endothelial denudation

Fan Yen Lee*, Hung I. Lu, Han Tan Chai, Jiunn Jye Sheu, Yi Ling Chen, Tein Hung Huang, Gour Shenq Kao, Sheng Yi Chen, Sheng Ying Chung, Pei Hsun Sung, Hsueh Wen Chang, Mel S. Lee, Hon Kan Yip

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

4 Scopus citations


This study tested the hypothesis that circulating microparticles (MPs) exacerbated vascular wall (VW) remodeling after endothelial denudation by 0.014 wire in a rat model. Adult male Sprague Dawley rats (n = 40) were equally categorized into group 1 [sham-control (SC); 3.0 mL saline intravenous injection], group 2 [SC + intravenous MPs (1.0 × 107) derived from patients with carotid artery stenosis (CAS)], group 3 [femoral arterial endothelial denudation (FAED)], group 4 (FAED + MPs derived from healthy subjects), and group 5 (FAED + CAS-derived MPs). Animals were euthanized by day 28 after FAED procedure. The results demonstrated that neointimal area (NIA) (mm2), medial area, and number of infiltrated cells in medial layer were highest in group 5 and lowest in groups 1 and 2, and significantly higher in group 4 than those in group 3 (all P<0.0001), but no differences were noted between groups 1 and 2. However, the ratio of luminal area to VW area showed an opposite pattern compared to that of NIA among five groups (P<0.0001). Immunofluorescent study showed an identical pattern of changes in the numbers of inflammatory (F4/80, CD14, CD40, IL-β) and proliferative (Ki-67, Cx43) cells in VW compared to that of NIA among the five groups (all P<0.00). The mNRA expressions of inflammatory (MMP-9, NF-κB, TNF-α, IL-1β, iNOS, PDGF) and cell activation (c-Fos, c-Myc, osteopontin, PCNA) biomarkers showed an identical pattern compared to that of NIA among all groups (all P<0.001). Take altogether, CAS-derived MPs further aggravated MP-mediated VW remodeling after endothelial damage compared to that observed after administration of MPS derived from healthy subjects.

Original languageEnglish
Article numberAJTR0027790
Pages (from-to)4511-4522
Number of pages12
JournalAmerican Journal of Translational Research
Issue number11
StatePublished - 2016

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© 2016, E-Century Publishing Corporation. All rights reserved.


  • Circulating microparticles
  • Endothelial denudation
  • Inflammation
  • Neointimal proliferation
  • Vascular wall remodeling


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