Circulating microRNA breast cancer biomarker detection in patient sera with surface plasmon resonance imaging biosensor

Chi Lok Wong, Sau Yeen Loke, Hann Qian Lim, Ghayathri Balasundaram, Patrick Chan, Bee Kiang Chong, Ern Yu Tan, Ann Siew Gek Lee*, Malini Olivo*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

17 Scopus citations

Abstract

In this article, we report for the first time, the detection of circulating miRNA as a breast cancer biomarker in patient sera using surface plasmon resonance imaging biosensor. The advantage of this approach lies in the rapid, label-free and sensitive detection. The sensor excites plasmonic resonance on the gold sensor surface and specific DNA-miRNA molecular bindings elucidate responses in the plasmonic resonance image. Experiments of detecting synthetic miRNA molecules (miR-1249) were performed and the sensor resolution was found to be 63.5 nM. The sensor was further applied to screen 17 patient serum samples from National Cancer Centre Singapore and Tan Tock Seng Hospital. Sensor intensity response was found to differ by 20% between malignant and benign cases and thus forms, a potential and an important metric in distinguishing benignity and malignancy.

Original languageEnglish
Article numbere202100153
JournalJournal of Biophotonics
Volume14
Issue number11
DOIs
StatePublished - 11 2021
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2021 Wiley-VCH GmbH

Keywords

  • SPR imaging
  • breast cancer biomarker
  • circulating miRNA
  • clinical study
  • surface plasmon resonance biosensor

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