Cloned cytotoxic T lymphocytes as target cells. II. Polarity of lysis revisited

K. Gorman, C. C. Liu, A. Blakely, J. D.E. Young, B. E. Torbett, W. R. Clark

Research output: Contribution to journalJournal Article peer-review

11 Scopus citations

Abstract

The original polarity of lysis experiments suggested that CTL are themselves sensitive to whatever mechanism it is that CTL use to lyse their targets. This concept has placed certain limitations on possible mechanisms of lysis by CTL. Recently, we found in studies with cloned CTL as targets that cloned CTL are in fact highly resistant to lysis by other CTL, as well as to their cytotoxic granule proteins. We show here that although cloned CTL are extremely resistant to lysis by primary and cloned CTL, they are readily inactivated functionally by all primary CTL and by at least one CTL clone. Moreover, cloned CTL are also functionally inactivated by cytotoxic granule proteins. The activity in granules responsible for functional inactivation of CTL, which we call inhibitin, is Ca2+ insensitive and distinct from hemolytic activity, and is, thus, unlikely to be perforin. These experiments suggest a possible alternative interpretation of the original polarity of lysis experiments.

Original languageEnglish
Pages (from-to)2211-2215
Number of pages5
JournalJournal of Immunology
Volume141
Issue number7
StatePublished - 1988
Externally publishedYes

Keywords

  • Animal
  • Clone Cells
  • Cytoplasmic Granules
  • Cytotoxicity Tests, Immunologic
  • Cytotoxicity, Immunologic
  • Female
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Inbred DBA
  • Peptides
  • Support, Non-U.S. Gov't
  • Support, U.S. Gov't, P.H.S.
  • T-Lymphocytes, Cytotoxic

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